甲状腺乳头状癌VEGF-C表达与血管、淋巴管生成的关系  

Expression of VEGF-C and angiogenesis,and lymphangiogenesis in papillary thyroid carcinoma

作　　者：梁青春[1] 魏启幼[1] 范松青[1] 

机构地区：[1]中南大学湘雅二医院病理科,长沙410011

出　　处：《中南大学学报（医学版）》2006年第3期414-416,419,共4页Journal of Central South University ：Medical Science

摘　　要：目的:探讨甲状腺乳头状癌(papillary thyroid carcinoma,PTC)中血管内皮生长因子(vascularendothelial growth factor C,VEGF-C)表达与血管、淋巴管生成的关系。方法:将72例PTC根据侵袭程度分为甲状腺乳头状微癌组、甲状腺内型乳头状癌组和甲状腺外型乳头状癌组;根据有无淋巴结转移分为淋巴结转移组和淋巴结无转移组。免疫组化SP法对PTC进行VEGF-C,CD105和血管内皮生长因子受体3(vascular endothelial growth factor receptor-3,VEGFR-3)标记,应用图像分析系统定量分析VEGF-C,计算出其阳性指数(positive index,PI)、显微镜下计数的微血管密度(microvessel density,MVD)和淋巴管密度(lymphaticvessel density,LVD)。结果:PTC淋巴结转移组VEGF-C的PI值(23.15±3.75)显著高于无转移组(14.54±2.93)(P<0.01);PTC微癌、腺内型、腺外型的MVD分别为35.25±2.06,41.75±5.46和52.58±4.16,随癌侵袭程度增加而增加(P<0.05);PTC微癌、腺内型、腺外型LVD分别为6.00±0.81,13.80±1.81和19.17±2.96,随癌侵袭程度增加而增加(P<0.05);淋巴结转移组的LVD(19.56±2.45)显著高于无转移组(12.48±2.84)(P<0.05);VEGF-C表达与MVD和LVD计数呈正相关(r=0.743,0.90,均P<0.01)。结论:VEGF-C,LVD与PTC的淋巴结转移有关;MVD,LVD与PTC侵袭有关;VEGF-C可能参与了血管、淋巴管生成。Objective To investigate the relationship between the expression of vascular endothelial growth factor C （VEGF-C）and angiogenesis and lymphangiogenesis in papillary thyroid carcinoma （PTC）. Methods Seventy-two PTC cases were divided into 3 groups according to the level of invasion： papillary microcarcinoma group （PMC group ）, intrathyroid carcinoma group （IPC group ） , and extrathyroid carcinoma group （ EPC group）. They were again divided into 2 groups according to lymph node metastasis： lymph node metastasis group and lymph node no-metastasis group. The expressions of VEGF-C, CD105 and vascular endothelial growth factor receptor-3 （VEGFR-3） were detected by SP method of immunohistochemical staining. The expression of VEGF-C was analyzed quantitatively by image analysis system, and the PI of VEGF-C （ VEGF-C-PI ） , the number of MVD （microvessel density ）, and LVD （lymphaticvessel density ） were obtained. Results The VEGF- C- PI of lymph node metastasis group （ 23. 15 ± 3.75 ） was higher than that of lymph node non-metastasis group （14. 54 ± 2. 93） （P 〈 0. 01）. MVD was 35. 25 ± 2. 06 in the PMC group, 41.75 ±5.46 in the IPC group, and 52.58 ± 4. 16 in the EPC group, which showed the elevatory tendency with the increase of invasion （ P 〈 0.05 ）. LVD was 6.00 ± 0.81 in the PMC group, 13.80 ± 1.81 in the IPC group, and 19. 17 ± 2.96 in the EPC group, which again showed the elevatory tendency with the increase of invasion （ P 〈 0.05 ）. The LVD of lymph node metastasis group （ 19. 56 ± 2. 45 ） was significantly higher than that of lymph node non-metastasis group （12.48 ± 2.84） （P 〈0.05 ）. VEGF-C was positively correlated with MVD and LVD （r =0.743, 0.90, P 〈 0.01 ）. Conclusion The expressions of VEGF-C and LVD are related to lymph node metastasis of PTC. MVD and LVD are related to the invasion of PTC. VEGF-C may play an important role in the angiogenesis and lymphangiogenesis.

关 键 词：甲状腺乳头状癌 血管内皮生长因子C 血管生成 淋巴管生成 免疫组化 

分 类 号：R736.1[医药卫生—肿瘤]