Characteristics of Ca^(2+)-exocytosis coupling in isolated mouse pancreatic β cells  

作　　者：Qian GE Yong-ming DONG Zhi-tao HU Zheng-xing WU Tao XU 

机构地区：[1]College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China [2]National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

出　　处：《Acta Pharmacologica Sinica》2006年第7期933-938,共6页中国药理学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(30270363,30470448 and 30130230);the National High Technology Research and Development Program of China(2004CB720000).

摘　　要：Aim： To characterize Ca^2＋-stimulated exocytosis in isolated mouse pancreatic β cells. Methods： An improved method was described for isolation of mouse pancreatic β cells by collagenase P. The Ca^2＋ channel current and the membrane capacitance were examined by using the whole-cell patch clamp recording technique. Results： Using depolarization and flash photolysis of caged Ca^2＋ to induce Ca^2＋-dependent exocytosis in β cell from KM mouse, we have explored the characteristics of the Ca^2＋ channel current and the relationship between Ca^2＋ signals and exocytosis. The averaged peak Ca^2＋ current measured at ＋20 mV was -60±6 pA （n=13）. Conclusion： We characterized three kinetically different pools of vesicles in mouse pancreatic β cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.Aim： To characterize Ca^2＋-stimulated exocytosis in isolated mouse pancreatic β cells. Methods： An improved method was described for isolation of mouse pancreatic β cells by collagenase P. The Ca^2＋ channel current and the membrane capacitance were examined by using the whole-cell patch clamp recording technique. Results： Using depolarization and flash photolysis of caged Ca^2＋ to induce Ca^2＋-dependent exocytosis in β cell from KM mouse, we have explored the characteristics of the Ca^2＋ channel current and the relationship between Ca^2＋ signals and exocytosis. The averaged peak Ca^2＋ current measured at ＋20 mV was -60±6 pA （n=13）. Conclusion： We characterized three kinetically different pools of vesicles in mouse pancreatic β cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.

关 键 词：MOUSE pancreatic β cells Ca^2＋-exocytosis coupling 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]