Injury of mitochondria and the expressions of fas and bax mRNA in the hippocampus of epileptic rats of different latency  

作　　者：Shuhai Tang Jianying Sun Xiaojun Pan Li Zhang 

机构地区：[1]Department of Anesthesiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China [2]Department of Neurology, Qilu Hospital of Shandong University, Jinan 250012, Shandong Province, China

出　　处：《Neural Regeneration Research》2006年第1期18-21,共4页中国神经再生研究（英文版）

基　　金：the Natural Science Foundation of Shandong Province, No. Y2001C10

摘　　要：BACKGROUND： It has been confirmed that Fas and Bax respectively mediate the exogenous and endogenous pathways of neuronal apoptosis, and then mediate the neuronal injury after status epilepticus. OBJECTIVE： To comparatively observe the injury of mitochondrial ultrastructure and the expressions of fas and bax in hippocampal tissue of rats with status epilepticus of different latency.DESIGN： A randomized control study.SETTING ： Department of Anesthesiology and Department of Neurology, Qilu Hospital of Shandong University. MATERIALS： Totally 110 male adult SD rats of 260-300 g were used. Kainic acid was purchased from American Sigma Company. METHODS： The experiment was carried out in the Pathological Laboratory of Shandong Academy of Medical Sciences between March and July 2005.① Totally 100 SD rats were divided into. two groups according to the random number table method： intraperitoneal injection group and caudal venous injection group. The rats were given kainic acid injected intraperitoneally （12 mg/kg） and through caudal vein （10 mg/kg） respectively. Each group was observed at 3, 6, 24, 48 and 72 hours after status epilepticus respectively. Ten rats were selected for each time point, including 2 for examination of electron microscope and 8 for the diction of the fas and bax mRNA expressions. The time and manifestations of seizure were observed, and the seizure was lasted for 2 hours, and then it was terminated by intraperitoneal injection of diazepam （10 mg/kg）. Another 10 rats were used as the normal control group, and the materials were taken at 24 hours after status epilepticus, 2 of rats for the examination of electron microscope and 8 of them for the reverse transcription-polymerase chain reaction （RT-PCR）.② The ultrastructure of hippocampal neurons and its mitochondria were observed with transmission electron microscope. ③ The fas and bax mRNA expressions were detected with reverse transcription-polymerase chain reaction （RT-PCR）. MAIN OUTCOME MEASURES： The compleBACKGROUND： It has been confirmed that Fas and Bax respectively mediate the exogenous and endogenous pathways of neuronal apoptosis, and then mediate the neuronal injury after status epilepticus. OBJECTIVE： To comparatively observe the injury of mitochondrial ultrastructure and the expressions of fas and bax in hippocampal tissue of rats with status epilepticus of different latency.DESIGN： A randomized control study.SETTING ： Department of Anesthesiology and Department of Neurology, Qilu Hospital of Shandong University. MATERIALS： Totally 110 male adult SD rats of 260-300 g were used. Kainic acid was purchased from American Sigma Company. METHODS： The experiment was carried out in the Pathological Laboratory of Shandong Academy of Medical Sciences between March and July 2005.① Totally 100 SD rats were divided into. two groups according to the random number table method： intraperitoneal injection group and caudal venous injection group. The rats were given kainic acid injected intraperitoneally （12 mg/kg） and through caudal vein （10 mg/kg） respectively. Each group was observed at 3, 6, 24, 48 and 72 hours after status epilepticus respectively. Ten rats were selected for each time point, including 2 for examination of electron microscope and 8 for the diction of the fas and bax mRNA expressions. The time and manifestations of seizure were observed, and the seizure was lasted for 2 hours, and then it was terminated by intraperitoneal injection of diazepam （10 mg/kg）. Another 10 rats were used as the normal control group, and the materials were taken at 24 hours after status epilepticus, 2 of rats for the examination of electron microscope and 8 of them for the reverse transcription-polymerase chain reaction （RT-PCR）.② The ultrastructure of hippocampal neurons and its mitochondria were observed with transmission electron microscope. ③ The fas and bax mRNA expressions were detected with reverse transcription-polymerase chain reaction （RT-PCR）. MAIN OUTCOME MEASURES： The comple

关 键 词：mRNA FIGURE 

分 类 号：R[医药卫生]