胃肠安对人胃癌细胞SGC-7901原位移植瘤细胞增殖和凋亡的影响  

Inhibition of Tumor Cell Proliferation and Induction of Apoptosis in Orthotopic Transplanted Gastric Cancer SGC-7901 by Wei Chang An

作　　者：郑嘉岗[1] 赵爱光[2] 顾缨[2] 尤圣富[3] 赵海磊[2] 唐莱娣[3] 

机构地区：[1]上海中医药大学附属龙华医院消化内科,200032 [2]上海中医药大学附属龙华医院肿瘤科 [3]上海中医药大学附属龙华医院科研实验中心

出　　处：《胃肠病学》2006年第6期336-339,共4页Chinese Journal of Gastroenterology

基　　金：上海市科委青年科技启明星计划(No.02QB14043);上海市教委科技发展基金(No.01C06)项目资助

摘　　要：背景:以健脾类中药为基础的复方胃肠安可抑制人胃癌细胞SGC-7901裸小鼠皮下移植瘤细胞的增殖,并诱导细胞凋亡,提高进展期胃癌的3年生存率。目的:通过观察胃肠安对人胃癌细胞裸小鼠原位移植瘤生长和凋亡的影响,探讨其抑制肿瘤的作用机制。方法:以高效液相色谱(HPLC)法监测复方胃肠安制剂的稳定性;采用胃小囊法,予45只雄性BALB/c-nu/nu裸小鼠接种人胃癌细胞SGC-7901移植瘤模型,造模第28天,选出扪及胃部肿块直径为3mm左右的裸小鼠共43只,随机分为胃肠安组(14只)、5-氟尿嘧啶(5-FU)组(14只)和对照组(15只);采用免疫组化SP法检测增殖细胞核抗原(PCNA)的表达;采用缺口末端标记(TUNEL)法检测细胞凋亡指数。结果:胃肠安组原位瘤抑制率为40.8%,肝转移率(7.7%)和总转移率(30.8%)显著低于对照组(50.0%和71.4%)(P<0.05);胃肠安组原位移植瘤PCNA阳性率和总阳性率(51.0%±11.4%和60.4%±13.3%)显著低于对照组(61.6%±11.6%和74.4%±7.9%)(P=0.0287和P=0.0029);胃肠安组细胞凋亡指数(17.3%±8.9%)显著高于对照组(7.8%±3.0%)(P=0.0010)。结论:复方胃肠安可抑制人胃癌细胞裸小鼠原位移植瘤的生长和转移,并可抑制人胃癌细胞原位移植瘤的细胞增殖和诱导凋亡。Background： Wei Chang An （WCA） decoction （Chinese herb medicine preparation） could increase the 3-year survival rate of patients with advanced gastric cancer and could enhance apoptosis and suppress tumor cell proliferation in human gastric cancer subcutaneous xenografts in nude mice. Aims： To appraise the inhibitory effects of Wei Chang An on the growth and metastasis of orthotopic transplanted gastric cancer SGC-7901 in nude mice and to study its effect on tumor cell proliferation and apoptosis induction. Methods： The stability of WCA preparation was monitored by HPLC. Orthotopic transplanted gastric cancer SGC-7901 model was used to evaluate the inhibitory effects of WCA on tumor growth and metastasis. Tumor cell proliferation was assessed by PCNA and apoptotic index was determined by TUNEL technique. Results： The tumor inhibition rate of WCA in this model was 40.8%. The total metastasis rate was 30.8% in WCA group, whereas it was 71.4% in the normal saline control group. The liver metastasis rate in WCA group was 7.7%, much lower than that in the control group （50.0%, P〈0.05）. The PCNA positivity rate and total positivity rate in the treatment group （51.0%±11.4% and 60.4%±13.3%） were significantly less than those in the control group （61.6%±11.6% and 74.4%±7.9%, P=0.0287 and P=0.0029）. The apoptotic index was much higher than that in the control group （17.3%± 8.9% vs. 7.8%±3.0%, P=0.0010）. Conclusions： WCA decoction has inhibitory effect on the growth and metastasis of orthotopic transplanted gastric cancer SGC-7901 in nude mice. The mechanism is probably related to its inhibitory effect on tumor cell proliferation and induction of apoptosis in vivo.

关 键 词：中草药 胃肠安 胃肿瘤 增殖细胞核抗原 细胞凋亡 肿瘤转移 

分 类 号：R735.2[医药卫生—肿瘤]