紫外分光光度法定量检测发酵液中紫杉烷类化合物  

Determination of Taxanes in Fungus Fermentation Liquid by UV

作　　者：金涛[1] 刘利群[2] 王伟[3] 平文祥[1] 周东坡[1] 

机构地区：[1]黑龙江大学生命科学学院生物制药系,哈尔滨150080 [2]黑龙江省药品检验所,哈尔滨150001 [3]东北农业大学食品学院,哈尔滨150030

出　　处：《黑龙江医药》2006年第4期248-249,共2页Heilongjiang Medicine journal

基　　金：黑龙江省"十五"重大攻关课题(GA02C101)黑龙江省教育厅资助项目(10533013)

摘　　要：目的:建立一种真菌发酵液中紫杉烷类化合物定量检测的紫外分光光度法。方法:真菌发酵液经乙酸乙酯提取后通过薄层层析制成供试品溶液,在241nm处测定紫外吸收值。结果:该检测方法具有良好的线性,精密度和较高的回收率。结论:是一种检测真菌发酵液中紫杉烷类化合物含量的有效方法。Objective： To establish a method for the content determination of taxanes in furgus fermentation liquid by UV, Methods： The ethyl acetate extraction of fungus fermentation liquid followed by thin layer chromatography was prepared as the sample which was detected at 241nm. Results：The detection method have good linearity, precision and high reoovery. Conclusion： It is effective detection method for taxanes in fungus fermentation liquid.

关 键 词：紫外吸收 紫杉烷 发酵 

分 类 号：R927.2[医药卫生—药学]