抗坏血酸和尿酸在胶束体系中的电化学行为及选择性测定的应用  

Electrochemical Behaviors of Ascorbic Acid and Uric Acid in Micellar Aqueous and Their Selective Determination

作　　者：彭娟[1] 高作宁[1] 

机构地区：[1]宁夏大学能源化工重点实验室化学化工学院,银川750021

出　　处：《分析化学》2006年第6期817-820,共4页Chinese Journal of Analytical Chemistry

基　　金：教育部科学研究重点项目(No.204149);宁夏自然科学基金(No.E1001)项目资助

摘　　要：以玻碳电极为工作电极,在PBS中用循环伏安法研究了抗坏血酸（AA）和尿酸（UA）在胶束体系中的电化学行为.在溴化十六烷基吡啶（CPB）胶束体系中,AA和UA的氧化峰电流增加,峰电位负移;在十二烷基苯磺酸钠（SDBS）胶束体系中,AA和UA的氧化峰电流减小,峰电位正移.在CPB中,AA和UA的氧化峰电位相差约270 mV,以此建立了AA和UA的同时测定方法.用微分脉冲伏安法测定AA和UA的氧化峰电流分别在1.0×10^-6～1.0×10^-2 mol/L和5.0×10^-7～1.0×10^-3 mol/L的范围内与各自的浓度范围呈良好的线性关系.在200倍AA共存时UA的检出限为5.0×10^-6 mol/L.此方法可应用于人体尿样中UA的测定,结果令人满意.The electrochemical behaviors of ascorbic acid （AA） and uric acid （UA） in miceUar aqueous solutions have been studied by cyclic voltammetry at glassy carbon electrode. The experimental results show that the anodic peak current of AA and UA in the cetylpyrid bromide （CPB） aqueous solutions, while it decreases in the sodium dodecyl benzene sulfonate （SDBS） aqueous solutions. The anodic peak potential of AA and UA shift more negative in CPB aqueous solutions, while it shifts more positive in SDBS aqueous solutions. The anodic peak of UA can be separated from AA by 270 mV in CPB aqueous solutions. Linear calibra- tion curves were obtained for AA and UA in ranges of 1.0 × 10^-6 - 1.0 × 10^-2 mol/L and 5.0 ×10^-7 - 1.0 × 10^-3mol/L respectively in CPB aqueous solutions by differential pulse voltammetry. The detection limit of UA in the presence of 200-fold excess of AA was 5.0 ×10^-6 mol/L. The method has been applied in determining the concentration of UA in human urine with satisfactory results.

关 键 词：抗坏血酸 尿酸 胶束 电分析方法 

分 类 号：O657.1[理学—分析化学]