葡多酚对辐射引发胰腺细胞Bcl-2和Bax蛋白异常表达的影响  

Effect of grape procyanidin on the abnormal expressions of Bcl-2 and Bax protein in pancreas cells irradiation with gamma rays

作　　者：刘海珍[1] 宋桂花[1] 钟进义[2] 

机构地区：[1]山东省潍坊市人民医院营养科,261041 [2]青岛大学医学院营养研究所

出　　处：《中华放射医学与防护杂志》2006年第3期215-217,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目(30271127)

摘　　要：目的探讨葡多酚(GPC)对亚慢性辐射引发的小鼠胰腺细胞凋亡及Bcl-2和Bax蛋白异常表达的抑制作用。方法给口服GPC的小鼠用60Coγ射线进行亚慢性辐照(每周5次,连续4周),末次照射后第2天处死动物,取胰腺组织用免疫组化染色试剂盒测定Bcl-2和Bax表达水平、透射电镜观察细胞超微结构的变化。结果GPC保护组Bcl-2表达率为51·1%,较辐射对照组升高;而Bax表达率则低于辐射对照组;细胞超微结构损伤较辐射对照组减轻。各项差异均有统计学意义(P<0·05)。结论GPC可抑制亚慢性辐射诱发的小鼠胰腺细胞凋亡及Bcl-2和Bax蛋白异常表达,对亚慢性辐射损伤有一定防护作用。Objective To investigate the effect of grape procyanidin （GPC）on the cell apoptosis and expressions of Bcl-2 and Bax protein in pancreas cells of mice irradiated with gamma rays. Method GPC was intragastrically administrated to the mice and then irradiated with ^60Co-γ rays 5 times per week for 4 weeks, dose was 2.5 Gy in total. All mice were executed on the second day after the last irradiation. Three indices including expression of Bcl-2 and Bax protein and uhrastructure of pancreas cells were examined. Result The Bcl-2 expression rate was 51.1% in the GPC protection group, higher than that in the irradiated control group. Bax expression, however, was lower than that in the irradiated control group. All the differences mentioned above were statistically significant （ P 〈0.01 ）. Injury to cell ultrastructure was alleviated in the GPC protection group. Conclusion The present study showed that GPC can inhibit radiation-induced cell apoptosis and abnormal expression of Bcl-2 and Bax protein in pancreas cells, and can prevent from chronic radiation injury.

关 键 词：辐射 酚类 胰腺细胞 BCL-2 BAX 

分 类 号：R818[医药卫生—放射医学]