α粒子和NNK联合作用的细胞遗传毒性  

Study on cellular genotoxicities induced by alpha particles irradiation in combination with NNK treatment

作　　者：李平[1] 杨陟华[1] 潘秀颉[1] 曹珍山[1] 米娜[1] 陈忠民[1] 刘刚[1] 魏菡[1] 李慧颖[1] 朱茂祥[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850

出　　处：《中华放射医学与防护杂志》2006年第3期229-232,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的研究α粒子和4-甲基亚硝基-1-(3-吡啶基)-1-丁酮(NNK)联合作用的细胞遗传毒性。方法永生化的人支气管上皮细胞(BEP2D细胞)分为正常对照组(NC)、α粒子单纯照射组(α)、NNK染毒组(NNK)、NNK染毒(100μg/ml)后α粒子照射组(NNK+α)和α粒子照射后NNK染毒(100μg/ml)组(α+NNK);用单细胞凝胶电泳法检测DNA损伤;用多核细胞法检测细胞次黄嘌呤鸟嘌呤核糖转移酶(HPRT)基因突变率;用细胞遗传学方法检测细胞微核率。结果与相同剂量NNK或α粒子单独作用比较,α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率均显著增高;扣除NNK效应后,α粒子和NNK联合作用诱发BEP2D细胞DNA损伤、HPRT基因突变率、以及细胞微核率也明显高于α粒子单独照射组。结论α粒子合并NNK的细胞遗传毒性具有协同性。Objective To investigate cellular genotoxicities of aplha particles irradiation in combination with NNK treatment. Methods Exponentially growing immortalized human bronchial epithelial cells were divided into the normal control group （NC）, alpha particles irradiation （α）, NNK administration group （NNK）,NNK administration （100 μg/ml） followed by alpha particles irradiation group （NNK ＋ α）, and alpha particles irradiation followed by NNK administration （100μg/ml） group （α ＋ NNK）. DNA damage were detected by single cell gel electrophoresis （ SCGE ） ; multinuclear cell assay was used to detect the frequency of the HPRT gene mutation; cell micronucleus frequency were detected by cytogenetic methods. Results In the group exposed to both alpha particles irradiation and NNK, DNA damage, HPRT gene mutation frequency, and cell micronucleus frequency were significantly higher than those in the same dose groups irradiated with alpha particles or NNK administration alone. Subtracted the NNK effect, DNA damage, HPRT gene mutation frequency and cell micronucleus frequency in the group irradiated by alpha particles in combination with NNK administration were significantly higher than those of alpha particles irradiation alone. Conclusion The genotoxicity of alpha particles irradiation in combination with NNK administration had synergistic effect.

关 键 词：α粒子照射 NNK BEP2D细胞 DNA损伤 HPRT基因突变 微核 

分 类 号：R734.2[医药卫生—肿瘤]