对继发于年龄相关性黄斑变性的脉络膜新生血管膜中肺炎衣原体的鉴定  

Identification of Chlamydia pneumoniae within human choroidal neovascular membranes secondary to agerelated macular degeneration

作　　者：KalayogluM.V. Bula D. Arroyo J. J. W. Miller 陈立军 

机构地区：[1]Retina Service, Massachusetts Eye and Ear Infirmary, Harvard Medical School, 243 Charles Street, Boston, MA 02114, United States

出　　处：《世界核心医学期刊文摘（眼科学分册）》2006年第5期32-33,共2页Digest of the World Core Medical Journals:Ophthalmology

摘　　要：Age-related macular degeneration (AMD) is a leading cause of blindness in the United States, and increasing evidence suggests that, it is an inflammatory disease. The prokaryotic obligate intracellular pathogen Chlamydia pneumoniae is emerging as a novel risk factor in cardiovascular disease, and recent seroepidemiological data suggest that C. pneumoniae infection is also associated withAMD. In this study,we examined choroidal neovascularmembrane (CNV) tissue from patients with neovascular AMD for the presence of C. pneumoniae and determined whether the pathogen can dysregulate the function of key cell types in ways that can cause neovascular AMD. Nine CNV removed from patients with neovascular AMD were examined for the presence of C. pneumoniae by immunohistochemistry (IHC) and polymerase chain reaction (PCR); in addition, we performed PCR on nine non-AMD eyes, and IHC on five nonAMD CNV, seven non-AMD eyes, and one internal limiting membrane specimen. Finally, human monocyte-derived macrophages and retinal pigment epithelial (RPE) cells were exposed to C. pneumoniae and assayed in vitro for the production of pro-angiogenic immunomodulators (VEGF, IL-8, and MCP-1). C. pneumoniae was detected in four of nine AMD CNV by IHC and two of nine AMD CNV by PCR, induced VEGF production by human macrophages, and increased production of IL-8 and MCP-1 by RPE cells. In contrast, none of the 22 non-AMD specimens showed evidence for C. pneumoniae. These data indicate that a pathogen capable of inducing chronic inflammation and pro-angiogenic cytokines can be detected in some AMD CNV, and suggest that infection may contribute to the pathogenesis of AMD.Age-related macular degeneration （AMD） is a leading cause of blindness in the United States, and increasing evidence suggests that, it is an inflammatory disease. The prokaryotic obligate intracellular pathogen Chlamydia pneumoniae is emerging as a novel risk factor in cardiovascular disease, and recent seroepidemiological data suggest that C. pneumoniae infection is also associated withAMD. In this study, we examined choroidal neovascularmembrane （CNV） tissue from patients with neovascular AMD for the presence of C. pneumoniae and determined whether the pathogen can dysregulate the function of key cell types in ways that can cause neovascular AMD.

关 键 词：脉络膜新生血管膜 年龄相关性黄斑变性 肺炎衣原体 聚合酶链反应(PCR) 细胞内病原体 鉴定 继发 新的危险因素 血清流行病学 细胞功能失调 

分 类 号：R779.6[医药卫生—眼科] R774.5[医药卫生—临床医学]