死亡相关蛋白激酶启动子区甲基化状态与膀胱癌的关系  

Association of CpG Island Methylation Status of Deathassociated Protein Kinase with Bladder Cancer

作　　者：赵敏[1] 李智[1] 于永春[2] 段建敏[3] 王红玲[2] 

机构地区：[1]同济大学附属第十人民医院检验科,上海200072 [2]同济大学附属第十人民医院中心实验室,上海200072 [3]兰州大学第二附属医院泌尿外科,甘肃兰州730030

出　　处：《同济大学学报（医学版）》2006年第3期29-32,共4页Journal of Tongji University(Medical Science)

摘　　要：目的探讨死亡相关蛋白激酶(death-associated protein kinase,DAPK)在膀胱癌中CpG岛的甲基化状态及其意义。方法收集40对新鲜的膀胱癌组织及其癌周正常组织;采用甲基化特异性PCR(methylation specific PCR,MSP)检测DAPK基因启动子区CpG岛的甲基化状态;并对MSP产物进行TA克隆。结果40对癌组织及其癌周正常组织中,7例癌组织检测到DAPK异常甲基化,1例癌周正常组织检测到异常甲基化(7/40vs.1/40,P>0.05);原发性与复发性膀胱癌中的甲基化阳性率分别为2/15、5/25,二者差异无显著性(P>0.05);在不同分级、分期膀胱癌中的差异均无显著性(P>0.05)。结论DAPK启动子在膀胱癌中的甲基化阳性率较低,并且与膀胱癌的分级、分期无相关性,因而在选择分子学指标辅助膀胱癌早期诊断时,DAPK的甲基化检测需慎用。Objective To investigate CpG island methylation status of death-associated kinase protein （DAPK） in bladder cancer and its meaning. Methods Forty fresh bladder tumor tissues and paired adjacent normal tissues were obtained from cystectomy specimens. The CpG island methylation status of DAPK was examined by means of methylation specific PCR （MSP）, and the products of MSP were subjected to TA cloning. Results seven of 40 tumor tissues showed aberrant methylation, as with 1 of 40 normal tissues （P 〉0.05）. The positive rate of DAPK methylation in primary and recurrent bladder cancer was 2/15, 5/25, respectively; there was no significant difference between them （P〉0.05）. In different staged or graded bladder cancer, there was no significant difference （P〉0.05）. Conclusion The positive rate of DAPK methylation is low in bladder cancer, and it does not correlate with grade and stage of bladder. Therefore, detection of DAPK methylation is not recommended in the diagnosis of bladder cancer with molecular markers.

关 键 词：膀胱癌 死亡相关蛋白激酶 甲基化特异性PCR 

分 类 号：R737.14[医药卫生—肿瘤]