抑癌基因PTEN在大肠癌癌变过程中表达的实验研究  

Expression of PTEN in the carcinogenesis of colorectal carcinoma

作　　者：黄海花[1] 杨少华[1] 吴秀浅[2] 郑志超[2] 

机构地区：[1]汕头大学医学院第二附属医院,广东汕头515041 [2]汕头大学医学院附属肿瘤医院,广东汕头515031

出　　处：《现代肿瘤医学》2006年第7期848-850,共3页Journal of Modern Oncology

基　　金：广东省汕头市科技基金资助项目(编号:汕科(府)1006号)

摘　　要：目的探讨抑癌基因PTEN的表达与大肠癌发生及浸润转移的关系。方法应用免疫组织化学EnVi-sion法原位观察正常大肠粘膜、癌旁单纯增生上皮、增生性息肉、腺瘤型息肉及癌组织中PTEN蛋白的表达。结果正常大肠粘膜均见PTEN蛋白的表达。癌旁单纯增生粘膜、增生性息肉、腺瘤型息肉及癌组织中PTEN蛋白的阳性表达率分别为92.3%、87.5%、70.6%及56.9%,其中早期癌的阳性表达率为66.7%,浸润癌的阳性表达率为55.9%。无淋巴结转移者的阳性表达率为57.5%,有淋巴结转移者为32.0%(P=0.0002)。PTEN蛋白阳性表达率随肿瘤分化程度降低而降低(P=0.028),随肿瘤Dukes分期的增加而下降,但无统计学意义(P>0.05)。结论PTEN蛋白的表达与大肠癌的发生、浸润及淋巴结转移有关,其低表达可能是大肠癌发生的一个信号。Objective： To explore the relationship between PTEN protein expression and the carcinogenesis of colorectal mucosa, invasion and metastasis of colorectal carcinoma. Methods： The expression of PTEN was detected in normal mucosa, simple hyperplastic mucosa, adenomatons polys, hyperplastic polyps, colorectal carcinoma by immunohistocbemistry （EnVision） technique. Results： Normal mucosa was all positive for PTEN protein expression. The positive expression rate of PTEN was 92.3% in simple hyperplastic mucosa, 87.5% in hyperplastic polyps, 70.6% in adenomatous polys and 56.9% in colorectal carcinomas. Its expression was lower in invasive carcinoma than in early carcinoma. Tumors with lymphatic metastasis showed significantly lower positive rate than those without lymphatic metastasis（ P = 0. 0002 ）. PTEN protein expression significantly correlated with cell differentiation （ P = 0. 028 ）, but did not associated with tumor Dukes stage. Conclusion： PTEN protein expression in colorectal carcinoma is related to cell differentiation, tumor invasiveness and lymph node metastasis. Its low expression may be the signal of the carcinogenesis of colorectal carcinoma.

关 键 词：PTEN 大肠肿瘤 浸润 转移 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]