紫杉醇诱导MCF-7乳腺癌细胞凋亡的蛋白质组研究  

Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells

作　　者：吴峰[1] 王清明[1] 范国才[1] 陈吉中[1] 陈惠鹏[2] 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]军事医学科学院生物工程研究所

出　　处：《中华肿瘤杂志》2006年第6期418-421,共4页Chinese Journal of Oncology

摘　　要：目的研究紫杉醇诱导MCF-7乳腺癌细胞凋亡的分子机制。方法以100 nmol/L紫杉醇作用MCF-7细胞24 h后提取细胞总蛋白,利用蛋白质组技术,对紫杉醇诱导的MCF-7乳腺癌细胞凋亡前后的细胞蛋白进行二维电泳图谱分析,通过质谱鉴定差异蛋白质。结果通过对凋亡前后的蛋白质组二维电泳图谱分析,找到了17个差异较大的蛋白质,其中6个蛋白质通过质谱得到了鉴定,它们是烯醇化酶、细胞核氯离子通道蛋白、角蛋白8、核糖体蛋白S12、galectin-1和Hint。结论通过蛋白质组技术,发现了在紫杉醇诱导MCF-7乳腺癌细胞凋亡前后发生变化的6种蛋白质；这些差异蛋白质在紫杉醇诱导细胞凋亡中发挥一定的作用。Objective To investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells. Methods In this study, the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis （2-DE）, and compared with those from untreated MCF-7 cells. The differential proteins were identified by mass spectrometry. Results At 24 hour after paclitaxel （ 100 nmol/L） treatment, MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map. Six of them were identified by mass spectrometry. They were enolase 1, chloride intracellular channel 1, keratin 8, ribosomal protein S12, galectin-1 and histidine triad nucleotide binding protein, respectively. Conclusion We effectively found the changed proteins in the process of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques. These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.

关 键 词：紫杉醇 MCF-7乳腺癌细胞 凋亡 蛋白质组 

分 类 号：R737.9[医药卫生—肿瘤] R338[医药卫生—临床医学]