叶酸代谢酶基因多态与结直肠癌易感性的关系  

Association between genetic polymorphisms in folate metabolic enzyme genes and colorectal cancer:a nested case-control study

作　　者：陈坤[1] 宋亮[1] 金明娟[1] 范春红[1] 蒋沁婷[1] 俞维萍[1] 

机构地区：[1]浙江大学公共卫生学院流行病与卫生统计教研室,杭州310031

出　　处：《中华肿瘤杂志》2006年第6期429-432,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金(30471492)

摘　　要：目的研究叶酸代谢酶基因多态MTHFRC677T、MTHFRA1298C、MTRA2756G和MTRRA66G及其联合作用与结直肠癌(CRC)易感性的关系。方法采用聚合酶链-限制性片段长度多态性方法,检测140例CRC患者和343例正常对照者的叶酸代谢酶基因多态,采用非条件Logistic回归模型和似然比检验,分析各多态及其联合作用与CRC的关系。结果MTR2756G等位基因携带者患CRC的风险是野生型纯合子(AA)的2倍(95% CI为1．22～3．40)。与同时是MTHFR1298AA和MTR2756AA基因型者相比,同时是MTHFRl298AA和MTR2756AG/GG基因型者患CRC的风险显著升高(OR=2．57,95%CI为1．42～4．65),两者之间存在联合作用(P=0．04)。结论MTR2756G等位基因可能是CRC的危险因素,MTHFRA1298C和MTRA2756G之间存在联合作用。Objective To investigate the interrelationship of genetic polymorphisms in folate metabolic enzymes（MTHFRC677T, MTHFRA1298C, MTRA2756G and MTRRA66G）and their combinative effects with colorectal cancer （CRC）. Methods A nested case-control study was designed and carried out. 140 CRC patients and 343 control subjects were included in this study. Polymorphisms of folate metabolic enzyme genes were genotyped by PCR-restriction fragment length polymorphism method. Risk of CRC was estimated by unconditional logistic model, and P value for interaction was calculated by likelihood test. Results The allele of MTR2756G showed a positive association with CRC （ OR = 2.04, 95% CI = 1.22 ～ 3.40）. Those with MTHFR1298AA and MTR 2756AG/GG genotypes had an elevated risk with CRC （ OR = 2.57, 95% CI, 1.42 ～ 4.65 ）, and their combinative effect showed a significant association with CRC （ P = 0.04）. Conclusion MTR2756G allele may be a risk factor of CRC, and interaction may exsit between polymorphisms of MTHFRA1298C and MTRA2756G. Further studies with larger sample and in different ethnic groups are needed.

关 键 词：叶酸代谢酶基因多态 MTHFRC677T MTHFRAl298C MTRA2756G MTRRA66G 结直肠癌 CRC易感性 

分 类 号：R735.3[医药卫生—肿瘤]