内质网分子伴侣糖调节蛋白94在大鼠酒精性肝损伤中的高表达和意义  

Expression of endoplasmic reticulum molecular chaperone GRP94 in rats with ethanol-induced liver injury

作　　者：龚作炯[1] 王鲁文 陈瑞[2] 张频[1] 

机构地区：[1]武汉大学人民医院感染科武汉大学病毒学国家重点实验室,430060 [2]华中科技大学同济医学院附属协和医院

出　　处：《肝脏》2006年第3期167-169,共3页Chinese Hepatology

摘　　要：目的检测酒精性肝损伤大鼠肝组织内质网分子伴侣糖调节蛋白94的表达情况,探讨酒精性肝损伤的发病机制。方法24只SD雌性大鼠分成对照组和模型组。模型组大鼠给予乙醇加鱼油灌胃配合高脂饮食8周,建立酒精性肝损伤模型,应用半定量逆转录聚合酶链反应(RT-PCR)法和免疫组化法检测大鼠肝组织内质网分子伴侣糖调节蛋白94mRNA和蛋白水平。结果与正常组比较,模型组大鼠肝组织糖调节蛋白94mRNA和蛋白表达明显增强(P<0.01)。结论酒精性肝损伤大鼠存在糖调节蛋白94高表达,这可能是该病的发病机制之一。Objective To detect the expressions of glucose regulated protein 94（GRP94） in rats with ethanol-induced liver injury, and study the mechanism of ethanol-induced liver injury. Methods 24 female SD rats were randomly divided into control and model groups. The model group rats were fed with high fat-contalning diet plus ethanol and fish oil gavage for 8 weeks. The control group rats were fed with normal diet and gavaged sodium chloride at the same does. At the end of 8 weeks, all rats were killed. Expression of GRP94 was detected by immunohistomistry and RT-PCR. Results Serum alanine aminotransferase and aspartate aminotransferase activity increased significantly compared to the control group （P 〈 0.01）.A significant elevation in the expression of GRP94 in liver of model group was found compared to the control group （ P 〈 0.01 ）. Conclusion GRP94 was activated by ethanol-induced injury. This information suggested that was involved in mechanism of ethanol-induced liver injury.

关 键 词：内质网分子伴侣 内质网应激 糖调节蛋白94 酒精性肝损伤 

分 类 号：R575[医药卫生—消化系统]