SD大鼠实验性变态反应性脑脊髓炎模型的建立  

Establishment of experimental allergic encephalomyelitis model in SD rat

作　　者：郑建筝[1] 高聪[1] 谢富华[1] 蒲蜀湘[1] 陈盛强[1] 

机构地区：[1]广州医学院第二附属医院神经内科,广州市510260

出　　处：《实用诊断与治疗杂志》2006年第7期469-472,共4页Journal of Practical Diagnosis and Therapy

基　　金：广东省科技攻关项目资助(项目编号2000216)

摘　　要：目的：探索SD大鼠实验性变态反应性脑脊髓炎模型制作的试验方法与技术。方法：采用完全福氏佐剂一脑脊髓匀浆，并辅以注射百日咳疫苗诱导SD大鼠实验性变态反应性脑脊髓炎模型，比较不同的免疫原（同种或异种），注射方法（一次或多次注射）以及性别差异对模型制作的影响。观察和比较动物的神经症状和病理改变。结果：使用同种脑脊髓匀浆，多次注射雌性SD大鼠诱导的模型，其发病持续时间分别为16～20d，10～14d；发病率为85％；复发率为46．67％，而致死率仅有5．88％；明显优于其他组别。病理学证实发病大鼠具有典型的脱髓鞘改变和胶质结节形成。结论：使用同种脑脊髓匀浆，多次注射雌性SD大鼠诱导的动物模型其临床表现、病理特征、病程特点与人类的多发性硬化具有很大的相似性，适用于多发性硬化的研究。Objective To explore the experimental method and technique of making experimental allergic encephalomyelitis model in SD rat. Methods The animal model was established in SD rat by injecting spinal cord homogenate in Freud＇s complete adjuvant and bordetella pertussis vaccine. Different rats were used to establish animal models with different methods such as homologous or heterologous homogenate, one or multiple injections. Its clinical symptoms and histological alteration were observed and compared. Results The animal model by injecting homologous homogenate twice in female SD rat was better than other, whose duration of paralysis was 16-20 clays and 10-14 days, and the incidence was 85%,the incidence of relapse was 46.67%,and the fatality rate was 5.88%. Many demyelinated lesions were found in the brain and spinal cord in animal like human with multiple sclerosis. Conclusion The animal model of experimental allergic encephalomyelitis induced by injecting homologous homogenate twice in female SD rat bears a resemblance to the features of multiple sclerosis, and might be a better model for the study on multiple sclerosis.

关 键 词：实验性变态反应性脑脊髓炎 SD大鼠 动物模型 

分 类 号：R744[医药卫生—神经病学与精神病学]