醇提板栗壳色素对羟自由基和超氧阴离子自由基的清除作用  

Effect of Pigment Extracted with Alcohol from Chestnut Shell on the Scavenging of Superoxide Anion and Hydroxyl Radicals

作　　者：何玲玲[1] 王新[2] 石中亮[1] 孙伶[1] 张英[1] 

机构地区：[1]沈阳化工学院应用化学学院,辽宁沈阳110142 [2]辽宁大学制药工程系,辽宁沈阳110036

出　　处：《安徽农业科学》2006年第10期2054-2054,2058,共2页Journal of Anhui Agricultural Sciences

摘　　要：以30%乙醇溶液从板栗壳中提取得到板栗壳色素CSPⅠ,并以Fenton反应产生.OH模型、邻苯三酚自氧化反应产生O2-.模型,用紫外和可见分光光度法试验了CSPⅠ对模型中产生的.OH和O2-.的清除作用。结果表明,CSPⅠ具有良好的清除.OH作用,且清除作用与色素浓度呈正相关性,EC50为75.65 mg/L;而CSPⅠ对O2-.没有明显的清除作用。The pigment noted as CSP was extracted from chestnut shell with 30 % alcohol/water. The scavenging effects on hydroxyl radicals and superoxide anion radicals of the pigment from chestnut shell were studied by UV-visible spectroscopy. The hydroxyl radicals and superoxide anion radicals are produced respectively in Fenton reaction and pyrogallol autoxidation system. The results showed that CSP I had the scavenging activity to hydroxyl radicals in a concentration -dependent manner. The scavenging capacity to hydroxyl radicals was represented by the concentration scavenging 50 % of all of radicals, which of CSP I was 75.65 mg/L. CSP I had no remarkable scavenging activity to superoxide anion radicals.

关 键 词：板栗壳色素 抗氧化活性 羟自由基 超氧阴离子自由基 清除作用 

分 类 号：S664.2[农业科学—果树学]