降钙素基因相关肽和神经生长因子对局灶性脑缺血再灌注大鼠空间学习记忆能力的影响  

Effects of calcitonin gene-related peptide and nerve growth factor on spatial learning and memory abilities of rats following focal cerebral ischemic-referfusion

作　　者：郑广顺[1] 杨涌杰[2] 方秀斌[3] 

机构地区：[1]厦门市第二医院神经外科,福建省厦门市361021 [2]中国医科大学附属第二医院神经外科,辽宁省沈阳市110004 [3]中国医科大学基础医学院神经生物学教研室,辽宁省沈阳市110001

出　　处：《中国临床康复》2006年第26期60-62,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:观察外源性降钙素基因相关肽和神经生长因子对局灶性脑缺血再灌注后大鼠学习记忆能力的影响。方法:实验于2005-02/07在中国医科大学基础医学院神经生物实验室进行,取清洁级8周龄雄性SD大鼠30只,抽签法随机分为假手术组、缺血再灌注组、降钙素基因相关肽和神经生长因子治疗组,每组10只。用线栓法制备大鼠大脑中动脉阻塞模型。2h后再次麻醉大鼠,直视下轻轻拔出栓线约10mm以实现再灌注。缺血再灌注组缺血2h后经腹腔注入生理盐水1mL;降钙素基因相关肽和神经生长因子治疗组缺血2h后经腹腔注入2mg/L降钙素基因相关肽0.5mL和1000U/mL神经生长因子0.5mL,1次/d,连续10d,第一次给药均在缺血再灌注后15min内完成。假手术组分离血管,不插栓线、不注射药物外,其余皆同于两组动物。按照ZeaLonga5级评分法进行神经功能评分。选取评分为1,2和3分的动物进行Morris水迷宫实验检测大鼠空间学习记忆能力变化,比较假手术组、缺血再灌注组、降钙素基因相关肽和神经生长因子治疗组间大鼠的空间学习记忆能力的差异。结果:实验过程中缺血再灌注组、降钙素基因相关肽和神经生长因子治疗组分别有3只和2只大鼠缺血再灌注后不符合模型判断标准,其余均进入结果分析。①造模10d后缺血再灌注组大鼠Morris水迷宫的隐匿平台逃避潜伏期明显比假手术组大鼠长(P<0.01);降钙素基因相关肽和神经生长因子治疗组大鼠隐匿平台逃避潜伏期明显比缺血再灌注组缩短(P<0.01)。②缺血再灌注组大鼠穿越原平台位置水域的次数明显比假手术组大鼠少[(1.79±0.39),(4.30±0.73)次/min,P<0.01];降钙素基因相关肽和神经生长因子治疗组大鼠穿越原平台位置水域的次数明显比缺血再灌注组大鼠多[(3.16±1.03),(1.79±0.39)次/min,P<0.01]。结论:降钙素基因相关肽和神经生长因子能显著改善大鼠大脑中动�AIM： To study the effects of exogenous calcitonin gene related peptide （CGRP） and nerve growth factor （NGF） on learning and memory abilities of rats with focal cerebral ischemic-reperfusion. METHODS： The experiment was conducted in Department of Neurobiology, Basic Medical College of China Medical University from February to July 2005.Totally 30 male SD rats of 8 weeks old were selected and randomly divided into sham-operation group, ischemic-reperfusion group and treatment group with CGRP and NGF （treatment group） with 10 rots in each group. Rat models of middle cerebral artery occlusion （MCAO） were established by method of occlusion, 2 hours after that rats were anesthetized and the thread was slightly drawn out for 10 mm under direct staring to perform reperfusion. Rats in the ischemic-reperfusion group received intraperitoneal injection of 1 mL of normal saline via the abdomen at two hours later, while rats in the treatment group at 2 hours later received intraperitoneal injection of 2 mg/L CGRP （0.5 mL） and 1 000 g/mL NGF （0.5 mL） once a day for 10 continuous days. First administration was accomplished within 15 minutes after ischemic-reperfuion. Rats in the sham-operation group were separated of the vessels without occlusion or administration. The neural function was evaluated with Zea Longa 5-grade scale. Animals with the score of one, two and three points were tested of the spatial learning and memory abilities by Morris Water Maze, and then compared with the shamoperation group, ischemic-reperfusion group as well as treatment group. RESULTS： Three and two rats in the ischemic-reperfusion group and treatment group respectively were not in accordance with the criteria in the process, and the rest were involved in the analysis of resuhs.①10 days after modeling, the escape latencies of rats in the ischemic-reperfusion group were obviously longer than those in the sham-operation group （P 〈 0.01）, and those were significantly shorter in the treatment group than in ischemic

关 键 词：脑缺血 降钙素基因相关肽 神经生长因子 鼠 学习 记忆 

分 类 号：R743[医药卫生—神经病学与精神病学]