大鼠视觉可塑性相关cDNA文库的构建及筛选  

Construction and screening of cDNA libraries related to visual cortex plasticity in rats

作　　者：江文珊[1] 阴正勤[1] 

机构地区：[1]第三军医大学西南眼科医院,重庆400038

出　　处：《眼视光学杂志》2006年第3期159-163,共5页Chinese Journal of Optometry & Ophthalmology

基　　金：国家杰出青年科学基金资助项目(30025014)

摘　　要：目的筛选视觉可塑性相关基因,探讨视觉可塑性关键期形成及终止的分子机制。方法应用抑制性消减杂交技术构建幼年和成年大鼠视皮层差异表达的cDNA文库,并通过PCR筛选、反向Northern杂交验证、测序及同源性检索对差异表达基因进行分析。结果成功建立了高消减效率的幼年和成年大鼠视皮层差异表达的cDNA文库,筛选得到42个在大鼠视觉可塑性关键期终止后视皮层上调表达的基因片段、51个在大鼠视觉可塑性关键期视皮层上调表达的基因片段。经测序及同源性分析,共得到16个有效序列,其中15个为已知基因,另一个可能为新基因片段。结论通过对幼年和成年大鼠视皮层差异表达的cDNA文库的建立,筛选出一批与视觉可塑性关键期形成或终止相关的候选基因,为进一步阐明视觉可塑性的分子机制提供了重要基础。Objective To screen genes associated with visual cortex plastieity in rats and investigate their formation and termination mechanisras at the molecular level during the critical period of visual cortex plasticity. Methods Subtractive cDNA libraries obtained from the visual cortex of juvenile and adult rats using a suppression subtractive hybridization technique were constructed. Using a PCR technique, reverse Northern hybridization, a sequencing and homology search was performed to analyze the different exprssions of gene fragments Results Juvenile and adult rat visual cortex subtractive cDNA libraries with high subtractive efficiency were successfully set up. Fortytwo up-regulated gene fragments were obtained from the visual cortex of adult rats whose critical period had already terminated. Fifty-one up-reguated gene fragments were obtained from the. visual cortex of juvenile rats. Sixteen sequences were, obtained by performing a sequencing and homology search. Of these, 15 were known gene fragments and one gene fragment was novel. Condusion A set of candidate genes associated with the formation or termination of the critical peried for visual cortex plasticity were screened from the subtracted cDNA library, establishing an important base for clarifying the mechanisms of visual cortex plasticity at the. molecular level.

关 键 词：视皮层 可塑性 CDNA文库 大鼠 

分 类 号：R77[医药卫生—眼科]