The effect of anastrozole on mRNA expression of oestrogen related gene in MCF-7 breast cancer cells  

作　　者：宋张骏 吴怡 马清涌 

机构地区：[1]Department of Hepatobiliary Surgery,First Hospital,Xi'an Jiaotong University,Xi'an 710061,China [2]Department of Obstetrics and Gynaecology,Fourth Hospital of Xi'an City,Xi'an 710004,China

出　　处：《Journal of Medical Colleges of PLA(China)》2006年第3期178-181,共4页中国人民解放军军医大学学报（英文版）

摘　　要：Objective: To look for additional markers of molecular biology response to anastrozole, a new aromatase inhibitor, on the growth and mRNA expression level of MCF-7 cell. Methods: We investigated the effect of anastrzole on growth and gene expression in the human breast cancer cell line MCF-7 and compared with the most widely used antiestrogen tamoxifen. We chose 4 genes to examine regulation of gene expression of estrogen regulated genes: PR A, PR B, ErbB-2 and cyclin D1. Results: Compared with the tamoxifen, a statistically significant growth inhibition was observed with anastrozole. The PR A, PR B and cyclin D1 mRNA level in anastrozole treated cells was sigificantly below the level in tamoxifen treated cells (P<0. 05). They had agonistic effect on ErbB gene (P>0. 05). Conclusion: The third generation of aromatase inhibitors anastrozole exert more inhibit function in some expression of estrogen regulated genes than tomoxifen in MCF-7 cell line.Objective： To look for additional markers of molecular biology response to anastrozole, a new aromatase inhibitor, on the growth and mRNA expression level of MCF-7 cell. Methods： We investigated the effect of anastrzole on growth and gene expression in the human breast cancer cell line MCF-7 and compared with the most widely used antiestrogen tamoxifen. We chose 4 genes to examine regulation of gene expression of estrogen regulated genes ： PR A, PR B, ErbB-2 and cyclin D1. Results： Compared with the tamoxifen, a statistically significant growth inhibition was observed with anastrozole. The PR A, PR B and cyclin D1 mRNA level in anastrozole treated cells was sigificantly below the level in tamoxifen treated cells （P〈0. 05）. They had agonistic effect on ErbB gene （P〉0. 05）. Conclusion： The third generation of aromatase inhibitors anastrozole exert more inhibit function in some expression of estrogen regulated genes than tomoxifen in MCF-7 cell line.

关 键 词：aromatase inhibitor competitive RT-PCR mRNA expression 

分 类 号：R737.9[医药卫生—肿瘤]