脂质体转染survivin反义寡核苷酸对K562细胞增殖及凋亡的作用  

Effect of liposomal transfection of survivin antisense oligonucleotide on K562 cell proliferation and apoptosis

作　　者：杨敏[1] 戚之琳[1] 齐世美[1] 陈艳萍[1] 毕富勇[1] 

机构地区：[1]皖南医学院生化教研室

出　　处：《临床肿瘤学杂志》2006年第6期414-418,共5页Chinese Clinical Oncology

基　　金：安徽省科学技术厅重点科研基金项目(200101023023)

摘　　要：目的:观察脂质体转染survivin反义寡核苷酸(ASODN)对白血病细胞K562的增殖及凋亡的影响。方法:人工合成survivin硫代ASODN,通过脂质体转染K562细胞,倒置显微镜下观察细胞形态学变化;用MTT法观察转染前后对细胞增生的影响;用细胞凋亡检测法(POD)观察细胞凋亡;RT-PCR检测survivin mRNA的表达。结果:(1)survivin ASODN抑制K562细胞增殖呈时间和剂量依赖性;(2)survivin ASODN可诱导K562细胞的凋亡,凋亡指数呈浓度依赖性;(3)survivinASODN处理组K562细胞survivin mRNA的表达水平显著下降。结论:脂质体转染survivin ASODN能够明显抑制K562细胞增殖,且能诱导细胞凋亡。survivin可能成为肿瘤基因治疗的一个新靶点。Objective： To explore the effect of liposomal transfection of survivin antisense oligodeoxynucleotide （ ASODN ） on leukemic cell lines K562 cell proliferation and apoptosis. Methods： Anti-survivin phosphorothioate ASODN was synthesized and trans- fected into K562 cells by liposome, cell morphological transformation was observed with inverted microscope;The effect on cell proliferation was examined by MTY assay. Cell apoptosis was ditermined in situ cell apoptosis detection kit（POD）. Survivin mRNA expression was determined by RT-PCR. Results： （ 1 ） Survivin ASODN inhibited K562 cells proliferation in a dose and time dependent manner. （2） Survivin ASDON can induce the cell apoptosis and the apoptotic index is in a dose dependent manner. （3）The expression of survivin mRNA significantly decreased after treatment with surviving ASODN. Conclusion：Survivin ASODN is able to inhibit the proliferation of leukemic cells lines K562 cell and induce the cell apoptosis, which might provide a new target for tumor gene therapy.

关 键 词：脂质体 SURVIVIN 白血病 细胞凋亡 K562细胞 

分 类 号：R730.3[医药卫生—肿瘤]