高温合并顺铂对人肺腺癌细胞株H1299的协同杀伤作用  

Synergistric effect of hyperthermia and cisplatin on human lung adenocarcinoma cell line H1299

作　　者：马胜林[1] 陈雪琴[2] 牟翰舟[1] 冯建国[1] 

机构地区：[1]浙江省肿瘤医院暨肿瘤研究所 [2]浙江中医药大学,310000

出　　处：《临床肿瘤学杂志》2006年第6期427-430,共4页Chinese Clinical Oncology

摘　　要：目的:观察高温合并顺铂对人肺腺癌细胞株H1299的协同杀伤作用。方法:用CCK-8法检测高温处理或未处理的细胞对顺铂的敏感性。单独42℃作用2h、顺铂10μg/ml作用24h和两者联合作用后,采用CCK-8法检测细胞抑制率和流式细胞仪分析细胞各时相DNA含量,观察两者对细胞毒性和细胞周期的影响。结果:高温作用后,细胞对顺铂的半数抑制浓度(IC50)从9·51μg/ml降低至6·07μg/ml;高温和顺铂联合作用对细胞有明显的协同杀伤作用,流式细胞检测也显示高温对细胞周期有明显的影响,G0/G1期细胞比例明显增加,S期细胞比例明显下降。结论:高温可以明显提高H1299细胞对顺铂的敏感性。Objective：To observe the synergistic effect of hyperthrnia and cisplatin on human lung adenocarcinoma cell line H1299. Methods ：The cells treated 42 degree for 2 hours or not were detected cisplatin sensitivity with CCK-8 assay. After 42 degree for 2 hours, ciplatin 10μg/ml for 24 hours or the combination with both sides, the inhibition ratio was detected wtih CCK-8 assay and flow cytometry analyzed DNA contents in every phase of cell proliferation, and we observed their synergistic effect on cytotoxicity and cell cycel. Results：After hyperthermia, the IC50 values to cisplatin decreased from 9. 51μg/ml to 6. 07μg/ml. CCK-8 assay showed hyperthermia and ciplatin had synergistic cell killing effect, and FCM also showed hyperthermia had significant effect on cell cycle with increase of G0/G1 phase population and reduction of S phase population. Conclusion：Hyperthermia can significantly increase the sensitivity of H1299 cells to cisplatin.

关 键 词：高温 顺铂 肺腺癌细胞 细胞毒性 细胞周期 

分 类 号：R730.53[医药卫生—肿瘤] R734.2[医药卫生—临床医学]