顺铂与卡铂对宫颈癌化疗作用的对照研究  

THE COMPARISION OF STUDY BETWEEN CISPLATIN AND CARBOPLATIN IN CHEMOTHERAPY OF CERVICAL CANCER

作　　者：钱晓蕾[1] 彭芝兰[2] 胡敏[2] 牛晓宇[2] 陈悦悦[2] 

机构地区：[1]中国福利会国际和平妇幼保健院妇产科,上海200030 [2]四川大华西第二医院妇产科

出　　处：《现代预防医学》2006年第7期1131-1132,1138,共3页Modern Preventive Medicine

摘　　要：目的：宫颈癌的化疗是以铂类药物为基础的化疗，对顺铂和卡铂在宫颈癌化疗中的作用进行体外药敏实验，对其细胞毒性进行比较，为临床选择用药提供实验依据。方法：1．ATP标准曲线的测定；2．采用三磷酸腺苷生物发光法，对宫颈癌细胞株Hela、Caski、Siha进行药敏实验，药物分别为顺铂和卡铂，浓度分别为0．5×、1×和2×血浆峰值浓度。结果：1．ATP标准品在浓度为10^-9～10^-5mol／ml时，与发光计数值显示极好的线性关系，直线方程为Y=0．892X＋10．257，相关系数r=0．998（P〈0．001）。2．在0．5×、1×和2×血浆峰值浓度下，Hela细胞株顺铂作用抑制率分别为53．5％、69．2％、100％，卡铂作用抑制率分别为56．1％、64．2％、98％；Siha细胞株顺铂作用抑制率分别为78．3％、84％、95％，卡铂作用抑制率分别为69．5％、77.8％、98％；Caski细胞株顺铂作用抑制率分别为36％、45％、52．6％，卡铂作用抑制率分别为40％、41．7％、42．3％，经统计学处理差异无统计学意义（P〉0．05）。结论：顺铂和卡铂对宫颈癌细胞株的细胞毒性作用无差异统计学意义，由于卡铂毒副作用小，在宫颈癌化疗中优于顺铂。Objective： To evaluate the effect of cisplatin and carboplatin using chemosensitivity testing, and compare their cell toxicity and provide experimental evidence for clinical application. Methods- 1.The mensuration of ATP standard curve. 2.An ATP - CVA test was used to determine the drug sensitivity of Hela calls, Caski calls, Siha cells. The drug were cisplatin and carboplatin and the concentration were 0.5 × PPC, 1 × PPC and 2 × PPC respectively. Results： 1. In a range of 10^-9 - 10^-5 mol/ml, there is a linear relationship between the log ATP and the log luminescence （Y = 0.892X ＋ 10.257, correlation coefficient r = 0. 998） （P 〈 0.001）. 2. At the concentration of 0.5 ×PPC, 1 ×PPC and 2 ×PPC, the restraint rate of clsplatin of Hela cells were 53.5 %, 69.2 % and 100 % respectively, the restraint rate of carboplatin of Hela cells were 56.1%, 64.2 % and 98 % respectively; the restraint rate of cisplatin of Siha cells were 78.3%, 84% and 95% repectively, the restraint rate of carboplatin of Siha cells were 69.5%, 77.8% and 98% respectively; the restraint rate of cisplatin of Caski cells were 36%, 45% and 52.6%, respectively, the restraint rate of carboplatin of Caski cells were 40%, 41.7% and 42.3%, respectively; the differences were not significant （P〉0.05）. Conclusion： The difference of cell toxicity between cisplatin and carboplatin was not significant, and carboplatin is better than cisplatin in the chemotherapy of cervical cancer for its little side effect.

关 键 词：宫颈癌 药物敏感试验 三磷酸腺苷生物发光法 顺铂 卡铂 

分 类 号：R737.35[医药卫生—肿瘤]