β1整合素反义寡核苷酸对人胰腺癌BXPC-3细胞体外侵袭力的影响  

Effect of integrin-β1 antisense oligodeoxynucleotides on invasive ability of pancreatic carcinoma BXPC-3 cells in vitro

作　　者：黄涛[1] 冯延平[1] 常青[1] 高军[1] 杜志勇[1] 秦仁义[1] 裘法祖[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院外科

出　　处：《中华实验外科杂志》2006年第7期834-836,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察β1整合素反义寡核苷酸(ASODN)对人胰腺癌BXPC-3细胞体外侵袭力的影响。方法采用脂质体将不同浓度β1整合素ASODN转染到人胰腺癌BXPC-3细胞中,逆转录-聚合酶链反应(RT-PCR)、Western印迹法和Transwell侵袭室方法分别检测细胞β1整合素mR- NA、蛋白表达水平及体外侵袭能力。结果与对照组相比,32～128mg/L ASODN转染后可抑制81整合素mRNA和蛋白的表达(P＜0．05),64mg/L ASODN转染使BXPC-3细胞体外侵袭力明显下降(P＜0．05)。结论靶向β1整合素的ASODN可有效抑制其在人胰腺癌BXPC-3细胞中的表达,并降低癌细胞体外侵袭力。Objective To investigate the effect of integrin-β1 antisense oligodeoxynucleotides （A- SODN） on invasive ability of pancreatic carcinoma cell line BXPC-3 in vitro. Methods Different concentrations of integrin-β1 ASODN were transfeeted into BXPC-3 cells with liposome. The expressions of integrin-β1 mRNA and protein was determined by RT-PCR and Western-blot. The invasive ability was measured by the number of cells to penetrate polycarbonates coated with matrigel. Results In contrast with the control group, the expressions of integrin-β1 mRNA and protein was significantly decreased after, transfeeted with 32-128 mg/L ASODN （P〈0.05） ; Invasive tests in vitro also showed that the invasive ability of BXPC-3 ceils was inhibited by transfectlon with 64 mg/L integrin-β1 ASODN. Conclusion In this cell line model, integrin-β1 ASODN could effectively inhibit the expression of integrin-β1 gene, thus inhibit the protein synthesis, and decrease the ability of invasion.

关 键 词：寡核苷酸类 反义 Β1整合素 侵袭 胰腺肿瘤 

分 类 号：R735.9[医药卫生—肿瘤] R735[医药卫生—临床医学]