发育中的海马神经元缺氧后N-甲基-D-门冬氨酸受体通道电流变化及腺苷干预作用  

Changes of N-Methyl-D-Aspartate-Receptor-Channels Current in Developing Hippocampal Neurons after Hypoxia and Effect of Adenosine Intervention

作　　者：李振彪[1] 吴希如[1] 姜玉武[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《实用儿科临床杂志》2006年第12期754-755,782,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金项目资助(39900160)

摘　　要：目的研究缺氧对发育中的海马神经元N-甲基-D-门冬氨酸(NMDA)受体通道电流的影响及腺苷干预作用的离子通道机制。方法应用膜片箝技术分别对培养日龄为61、6 d的海马神经元缺氧前、后NMDA受体通道电流进行全细胞记录,测定其电流的最大幅值并研究腺苷对其干预作用。结果在缺氧状态下,培养日龄为6 d的海马神经元,其NMDA受体通道电流最大幅值与正常对照组相比显著升高(P<0.05)。培养日龄为16 d的海马神经元,其NMDA受体通道电流最大幅值与正常对照组相比亦显著升高(P<0.001)。予腺苷干预则使缺氧状态下培养日龄为6 d的海马神经元NMDA受体通道电流最大幅值显著降低(P<0.01)。而培养日龄为16 d的海马神经元,腺苷干预后其NMDA受体通道电流最大幅值无明显变化。结论缺氧使海马神经元NMDA受体通道电流幅值升高。腺苷通过降低NMDA受体通道电流幅值,对未成熟的海马神经元缺氧性损伤有一定的保护作用。Objective To study the changes of N - methyl - D - aspartate （NMDA） - receptor - channels current in developing hippocampal neurones during hypoxia and effect of adenosine intervention. Methods Whole - cell patch - clamp techniques cultured hippocmpal neurons whose cultured day were 6 days and 16 days respectively, the amplitude of the NMDA- receptor - channels currents of hippocampal neuron were determined. And the effect of hypoxia on the NMDA - receptor - channels current, and adenosine regulatory mechanisms in cultured hippocampal neurons were explored. Results During hypoxia, compared with control group, the amplitude of the NMDA- receptor - channels currents of hippocampal neuron whose cultured day was 6 days were significantly increased （ P 〈 0.05 ）. Moreover, the amplitude of the NMDA - receptor - channels currents of hippocampal neuron whose cultured day was 16 days were also significantly increased（ P〈0. 001 ）. During hypoxia, adenosine could significantly decrease the amplitude of the NMDA - receptor - channels currents of hippocampal neuron whose cultured day was 6 days, whereas, the amplitude of the NMDA- receptor- channels current of hippocampal neuron whose cultured day was 16 days was not decreased obviously by adenosine. Conclusions The results suggest that NMDA - receptor - channels in rat hippoeampal neurons are activated significantly during hypoxia. During hypoxia, the NMDA - recep- tor - channels current of immatured hippocampal neuron is decreased obviously by adenosine, which may be related to the suppress of adenosine to the increase of ealcium in cells.

关 键 词：全细胞记录 海马 神经元 发育 N-甲基-D-门冬氨酸-受体通道 缺氧 

分 类 号：R363[医药卫生—病理学]