降低聚腺苷二磷酸核糖聚合酶活性对外源癌基因诱导的NIH3T3转化的逆转作用研究  

EFFECT OF LOWERING POLY(ADP-RIBOSE)POLYMERASE ACTIVITY ON REVERSION OF NIH3T3 TRANSFORMATION INDUCED BY AN EXOGENOUS ONCOGENE

作　　者：杨其伟[1] 董元舒[1] 陈雅文[1] 陈德凤[1] 

机构地区：[1]南开大学分子生物学研究所

出　　处：《南开大学学报（自然科学版）》1996年第3期35-39,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金

摘　　要：用ＰＡＲＰ酶抑制剂苯甲酰胺（ＢＡ）处理经ｃ－Ｈａ－Ｔ２４－ｒａｓ活化癌基因转染的ＮＩＨ３Ｔ３细胞得到转化细胞系，结果表明：经苯甲酸胺处理的细胞系，其细胞凝集、血清依赖性、形态特征等均发生了改变，呈现出正常细胞的特征．Ｓｏｕｔｈｅｒｎ杂交结果表明，已整合到细胞基因组中的外源Ｔ２４～ｒａｓ基因发生了丢失．A transformant obtained by transfection of NIH3T3 cells with c-Ha-T24-ras oncogene was treated with benzamide,an inhibitor of PARP enzyme. Some changes including the properties of cell agglutination, serum dependence, morphology for the transformant line were ob served. Southern blot analysis showed that the exogenous c-Ha-T24-ras gene which had been integrated into genomic DNA of recipient cells was deleted.

关 键 词：苯甲酰胺 细胞逆转 PAR NIH3TE细胞 癌基因 

分 类 号：R730.231[医药卫生—肿瘤]