镰刀菌毒素丁烯酸内酯对软骨细胞分化的影响及硒的保护效应  

Toxic Effect of Butenolide on Chondrocyte Differentiation and the Protective Effect of Selenium

作　　者：左弘 郭雄 王世捷 师钟丽 彭双清[2] 曹峻岭[2] 张增铁[2] 

机构地区：[1]Key Laboratory of Environment and Genes Related Diseases of Ministry of Education,Institute of EnvironmentandEndemic Diseases,XianJiaotong University,Xian710061 [2]军事医学科学院毒物药物研究所

出　　处：《中国医学科学院学报》2006年第3期382-385,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30340004)~~

摘　　要：目的研究镰刀菌毒素丁烯酸内酯(BUT)对软骨细胞分化的影响及硒(Se)的保护作用。方法体外单层及立体培养软骨细胞,分为对照组(不作任何处理)、加Se0·1μg/ml对照组、BUT0·1μg/ml组、BUT1·0μg/ml组、BUT5·0μg/ml组和BUT1·0μg/ml+Se0·1μg/ml组等6个处理组。采用单克隆、多克隆抗体免疫组织化学法,观察各种处理因素对Ⅱ型胶原(ColⅡ)、X型胶原(ColX)、碱性成纤维细胞生长因子(bFGF)及甲状旁腺激素相关多肽(PTHrP)在培养软骨细胞中表达的影响。结果中、高剂量BUT组Ⅱ型胶原表型表达阳性率明显低于对照组(P<0·05);BUT+Se组Ⅱ型胶原表型表达阳性率明显高于中、高剂量BUT组(P<0·05);低、中、高剂量BUT组培养软骨细胞的bFGF和PTHrP的表达显著增高(P<0·05);各组均未见X型胶原的表型表达。结论BUT可影响软骨细胞Ⅱ型胶原的合成,补硒对BUT损伤软骨细胞有一定的保护作用。Objective To study the effect of butenolide （BUT） on cultured chondrocytes differentiation and the possible protective effects of selenium （Se）. Methods Ex-vivo cultured chondrocytes were divided into six groups： （1） Control group （without BUT and Se） ; （2） Se 0. 1μg/ml control group; （3） BUT 0. 1μg/ml group; （4） BUT 1.0μg/ml group; （5） BUT5. 0μg/ml group; and （6） BUT 1.0μg/ml ＋Se 0. 1μg/ml group. The expression of collagen Ⅱ （ Col Ⅱ ） , collagen X （ColX） , basic fibroblast growth factor （bFGF）, and parathyroid hormone-related peptide （PTHrP） in （or around） chondrocytes in all groups were analyzed by immunohistochemistry. Results The expressions of Col Ⅱ in 1.0 μg/ml BUT group and 5.0μg/ml BUT group were significantly lower than those in the control group （ P 〈 0. 05 ）. The expression of Col Ⅱ in 1.0 μg/ml BUT ＋ Se group were significantly higher than those in the 1.0 μg/ml BUT group and 5.0 μg/ml BUT group （P 〈 0. 05 ）. The expressions of bFGF and PTHrP of BUT groups were significantly higher than those in the Se and control groups （ P 〈 0.05 ）. No expression of ColX was observed in all groups. Conclusion BUT can affect the collagen Ⅱ synthesis of the chondrocytes. Selenium supplementation may play a protective role.

关 键 词：镰刀菌毒素 丁烯酸内酯 软骨细胞分化 硒 

分 类 号：R599.9[医药卫生—内科学]