苦参碱抑制人肾癌细胞系GRC-1细胞株增殖和促凋亡的实验研究  

Effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1

作　　者：种铁[1] 牛建强[1] 王子明[1] 佘军军[1] 黄晨[2] 

机构地区：[1]西安交通大学第二医院泌尿外科,陕西西安710004 [2]西安交通大学环境与疾病相关基因教育部重点实验室,陕西西安710061

出　　处：《中西医结合学报》2006年第4期388-391,共4页Journal of Chinese Integrative Medicine

摘　　要：目的观察苦参碱对体外人肾癌细胞系GRC-1细胞株增殖及细胞凋亡的影响,探讨苦参碱诱导GRC-1细胞株凋亡的作用机制。方法应用不同浓度的苦参碱分别作用于人肾癌细胞系GRC-1细胞株24、48、72、96h。采用MTT法观察苦参碱对GRC-1细胞株的细胞毒作用;透射电镜和流式细胞术观察、检测苦参碱诱导的细胞凋亡;免疫细胞化学技术检测苦参碱对GRC-1细胞株Bcl-2、Bax蛋白表达的影响。结果不同浓度的苦参碱对GRC-1细胞株均有一定的增殖抑制作用,且呈量效和时效关系。苦参碱能明显诱导GRC-1细胞株凋亡。经苦参碱作用后,GRC-1细胞株Bcl-2蛋白表达明显减弱而Bax蛋白表达明显增强。结论苦参碱对体外人肾癌细胞系GRC-1细胞株有一定的增殖抑制作用,其机制可能与调节Bcl-2/Bax蛋白表达比值以诱导细胞凋亡相关。Objective： To observe the effects of matrine on proliferation and apoptosis of human renal cell carcinoma cell line GRC-1 in vitro, and to explore its mechanism. Methods. The human renal cell carcinoma cell line GRC-1 was treated with matrine of different concentrations for 24, 48, 72 and 96 h respectively. The MTT assay was used to evaluate the cytotoxic effects of matrine on GRC-1 cells. The transmission electron microscope and flow cytometry were utilized to observe and detect the apoptosis of GRC-1 cells induced by matrine. The expression levels of Bcl-2 and Bax proteins were evaluated by streptavidin-biotin-peroxidase method. Results： The matrine of different concentrations all have cytotoxic effects on GRC-1 cells, with obvious dose- and time-dependent effects. The apoptosis induced by matrine was confirmed in GRC-1 cells. With intervention of matrine （1.5g/L） for 12 h, the expression level of Bcl-2 in GRC-1 cells was decreased while the expression level of Bax was increased as compared with those in the untreated group. Conclusion： The proliferation-inhibiting effects of matrine on human renal cell carcinoma cell line GRC-1 may be related to down-regulating the ratio of Bcl-2/Bax protein expression and promoting the apoptosis.

关 键 词：苦参碱 肾肿瘤 细胞凋亡 

分 类 号：R737.11[医药卫生—肿瘤]