β-榄香烯对兔VX2肾移植癌的放射增敏作用  

Radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo

作　　者：佘军军[1,2] 王子明[1] 车向明[3] 潘承恩[3] 

机构地区：[1]西安交通大学医学院第二附属医院泌尿外科 [2]西安交通大学医学院第一附属医院普通外科,陕西西安710061 [3]西安交通大学医学院第一附属医院普通外科

出　　处：《中西医结合学报》2006年第4期392-396,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30371831)

摘　　要：目的观察β-榄香烯对兔VX2肾移植癌的放射增敏作用。方法建立兔VX2肾移植癌模型。将动物随机分为空白对照组、放疗组和放射增敏组(放射治疗结合β-榄香烯乳剂肾动脉灌注治疗)。予以各组相应干预措施后,采用螺旋CT及B超动态监测肿瘤体积的变化,比较各组肿瘤生长延缓时间,计算β-榄香烯乳剂的放射增敏比(sensitizationenhancementratio,SER)。同时,观察各组肿瘤组织的病理学改变。采用TdT介导的带生物素dUTP缺口末端标记法检测各组肿瘤细胞凋亡指数。结果采用6MevX线放射治疗(3Gy·Fx-1·d-1×5d)结合β-榄香烯乳剂肾动脉灌注治疗(10mg·kg-1·d-1×5d)的放射增敏组,其肿瘤的放射增敏效应最为显著。放射增敏组肿瘤生长延缓时间明显长于放疗组和空白对照组。β-榄香烯乳剂SER为1.89。透射电镜下放射增敏组肿瘤细胞凋亡较放疗组和空白对照组显著。放射增敏组肿瘤细胞凋亡指数明显高于放疗组和空白对照组。结论低剂量β-榄香烯乳剂对兔VX2肾移植癌具有放射增敏作用,可诱导肿瘤细胞凋亡。Objective： To investigate the radiosensitization of β-elemene on VX2 carcinoma transplanted on kidney in rabbits in vivo. Methods： The rabbits were all transplanted with VX2 carcinoma on kidney. The appropriate dose of β-elemene infusion via renal artery for further study on radiosensitization was determined. Then fifty-five rabbits were divided into three groups, untreated group, radiation group and radiation plus β- elemene-treated group. After corresponding intervention for each group, the tumor volume was measured by ultrasonography and spiral computed tomography, The sensitization enhancement ratio （SER） of β-elemene was calculated. The pathological change of tumor tissue in kidey was observed by light microscopy and electron transmission microscopy. The apoptotic index was also examined by TdT-mediated dUTP-biotin nick end labeling method. Results： The most significant radiosensitivity was observed in the radiation plus β- elemene-treated group with 6 Mev X-ray radiation dose of 3 Gy · Fx^-1· d^-1 × 5 d and β-elemene dose of 10 mg· kg^-1 · d^-1. The average time delayed for tumor growth was obviously longer in the radiation plus β- elemene-treated group than those in the untreated group and radiation group. The SER of β-elemene was 1.89. The apoptotic index of tumor cells in the radiation plus β-elemene-treated group was also significantly higher than those in the untreated group and radiation group. Conclusion： The β-elemene can enhance the effects of irradiation on VX2 carcinoma transplanted to kidney in rabbits in vivo by inducing apoptosis of tumor cells.

关 键 词：Β-榄香烯 兔VX2肾移植癌 放射增敏 细胞凋亡 

分 类 号：R737.11[医药卫生—肿瘤]