乳腺癌与其周围正常组织17β羟类固醇脱氢酶活性的比较  

ASSAY OF 17β-HSD ACTIVITY IN MALIGNANT HUMAN BREAST TISSUE

作　　者：田克立[1,2] 李涵[1,2] 于雪艳 王培林[1,2] 孔峰 胡晓燕[1,2] 徐松德[1,2] 

机构地区：[1]山东医科大学生物化学教研室 [2]山东医科大学附属医院普外科

出　　处：《山东医科大学学报》1996年第4期279-282,共4页Acta Academiae Medicinae Shandong

摘　　要：测定了１６例乳腺癌及癌旁正常组织标本的１７β羟类固醇脱氢酶（１７β－ＨＳＤ）活性和Ｅ２水平。结果显示，癌组织１７β－ＨＳＤ活性较正常组织明显下降，而Ｅ２水平升高。提示该酶活性及Ｅ２水平的改变与乳腺癌有关，但二者的变化无明显相关性（ｒ＝－０．３５４，Ｐ＞０．１）The 17β-hydroxysteroid dehydrogenase(17β-HSD)activity and concentrations of estradiol(E2)were determined in non-malignant and malignant human breast tissues. The radiolabelled estradiol was used as the substrate and the activity of 17β-HSD in 800g supernatant fraction was calculated as pmol estradiol converted to estrone/mg protein 30min-1. The substrate and product were separated with TLC method. The 17β-HSD activity was significantly lower(P<0. 001 )in malignant than that of non-malignant breast tissues, but estradiol levels were significantly higher (P<0. 001) in breast cancer. The authors failed to observe the significant correlation between enzyme activity and tissue levels of estradiol in breast cancer. These results indicate that the lower 17β-HSD activity, which altered interconversion of estrogens, and the increased estradiol concentrations in breast cancer tissues may provides estrogenic stimulus and enhance the growth and progression ofbreast cancer.

关 键 词：羟甾类脱氢酶类 乳腺癌 雌激素 

分 类 号：R737.902[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]