散发性结直肠腺癌APC、β-catenin基因的表达及其意义  

Expression of APC andβ-catenin Gene and Their Significance in Human Sporadic Colorectal Adenocarcinoma

作　　者：黄凯[1] 许东坡[2] 叶建新[2] 

机构地区：[1]江西省肿瘤医院腹部外科,330029 [2]福建医科大学附属第一医院肿瘤外科,350005

出　　处：《实用癌症杂志》2006年第2期134-136,共3页The Practical Journal of Cancer

基　　金：福建省教育厅科研基金资助项目(K2001081)

摘　　要：目的探讨APC、β-catenin在散发性结直肠肿瘤发生、发展中的作用和意义。方法采用SP免疫组化方法检测55例散发性结直肠腺癌、33例结直肠腺瘤组织标本中APC、β-catenin基因蛋白产物的表达水平。结果APC基因蛋白产物在结直肠腺癌组织中阳性表达率(23.6%)明显低于正常结直肠黏膜组织(86.7%)和结直肠腺瘤组织(72.7%),差异有显著性(P<0.05)。结直肠腺瘤、结直肠腺癌组织中β-catenin阳性表达率(69.7%、81.8%)明显高于正常结直肠黏膜组织(0%),差异有显著性P<0.05。结直肠腺癌中APC的表达与β-catenin的表达呈负相关关系γ=-0.514,P<0.05。结论APC、β-catenin基因在散发性结直肠腺癌的发生、发展过程中有重要作用。散发性结直肠腺癌组织中β-catenin细胞核聚集的主要原因可能是APC蛋白产物功能的失活。β-catenin细胞核异位表达是散发性结直肠腺癌发生发展过程中的早期改变。Objective To explore the role and value of APC and β-catenin in the carcinogenesis and progression of sporadic colorectal adenocarcinomas. Methods Immunohistochemical methods were used to detect the expression of APC andβ-catenin protein in 45 cases of normal colorectal mucosa, 33 cases of sporadic colorectal adenorna, 55 cases of sporadic colorectal carcinoma. Resuits ①The percentage of APC protein expression in colorectal adenocarcinomas（23.6 % ） was significantly lower than that in norrnal colorectal mucosa tissues（86.7 % ） and colorectal adenornas （72.7 % ） （P 〈 0.05）. ② The percentages of β-catenin nuclear expression in the colorectal adenomas （69.7%） and adenocarcinomas（81.8% ） were significantly greater than that in normal mucosa tissues（0） （P 〈 0.05 ）. （β）There was an inverse correlation between APC and β-catenin protein expression in eoloreetal adenocareinornas and there was no correlation between loss of APC and nuclear accumulation of β-catenin in colorectal adenomasγ= - 0. 514, P 〈 0.05. Conclusion ①APC andβ-catenin protein play an important role in carcinogenesis of sporadic colorectal adenocarcinomas. ②The major cause ofβ-catenin nuclear accumulation in colorectal tumors is dysfunction of APC. ③β-catenin nuclear expression in sporadic colorectal adenocarcinornas is an early alteration.

关 键 词：APC蛋白 Β-CATENIN蛋白 结直肠肿瘤 散发性结直肠肿瘤 免疫组织化学 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]