荧光定量PCR检测干旱胁迫下长春花Crlea基因的相对表达(英文)  

Comparative expression analysis of Crlea gene in Catharanthus roseus under drought stress by real-time quantitative PCR

作　　者：祖元刚[1] 聂明珠[1] 房思良[1] 于景华[1] 郭晓瑞[1] 

机构地区：[1]东北林业大学森林生态学教育部重点实验室,哈尔滨150040

出　　处：《植物研究》2006年第4期405-410,共6页Bulletin of Botanical Research

基　　金：Supported by the State Key Basic Researchand Development Plan of China(G19990160)

摘　　要：首次从长春花中克隆了Crlea（Crlea for Catharanthus roseus late embryogenesis abundant）的全长基因,采用荧光定量PCR方法对干旱胁迫下长春花叶片和根部Crlea基因的表达模式进行监测,结果表明,在0.5～8h的胁迫时间中,叶片和根部的Crlea基因表现出相似的积累模式.长春花Crlea基因的表达随着胁迫时间的延长而表达增强.在叶片中,在6 h和8 h的干旱处理后,Crlea基因表达显著提高,分别是未处理材料的9.984和20.431倍.在根部, 在8 h的处理后,Crlea基因的表达量显著提高（2.831倍于对照）.初步结果表明Crlea基因的表达没有组织特异性,并且为干旱胁迫正调控.A full-length Crlea（Crlea for Catharanthus roseus late embryogenesis abundant） gene was first isolated from Catharanthus roseus. Gene expression profiles of Crlea gene in leaves and roots under drought stress were monitored by real-time quantitative PCR. The results showed that a similar accumulation pattern of Crlea gene in leaves and roots over the observation period of 0.5 to 8 hours. The expression of Crlea mRNA was strengthened with the prolongation of stress time. In leaves, expression amounts of Crlea gene were 9.984 and 20.431 times higher than that of control respectively at 6 and 8 h. Similarly, the expression amounts of Crlea gene in root obviously increased （2.831 times higher than that of control） at 8 h. Primary results show the expression of Crlea gene is non-tissue-specific and up-regulated under drought stress.

关 键 词：长春花 干旱胁迫 晚期胚胎丰富蛋白 荧光定量 相对表达 

分 类 号：Q945.78[生物学—植物学] TS207.3[轻工技术与工程—食品科学]