T-AK细胞上清液抑瘤活性的研究  

Study on Antitumor Activities in Supernatant Preparations from T-AK Cells in Culture

作　　者：魏虎来[1] 赵怀顺[1] 贾正平[2] 辛夷[3] 

机构地区：[1]兰州医学院血液病研究所 [2]兰州军区总医院 [3]兰州医学院外科手术学教研室

出　　处：《兰州医学院学报》1996年第4期10-12,共3页Journal of Lanzhou Medical College

基　　金：甘肃省中青年科技基金(ZQ-93-14)

摘　　要：本文应用MTT比色法检测了rIL—2和抗CD_3单抗共同诱导激活的T—AK细胞培养上清液对K_(562)细胞和BEL—7402细胞增殖的抑制效应,并观察了T—AK上清液抑瘤活性与T—AK细胞杀伤活性的相关性。结果表明:①T—AK细胞能够自发地分泌一些抑制K_(562)细胞和BEL—7402细胞增殖的抑制因子,但活性很低;②T—AK细胞在杀伤K_(562)细胞过程中释放的抑制肿瘤细胞生长的物质,抑瘤活性较高,其作用为非特异性的;③T—AK细胞杀伤活性与其上清液的抑瘤活性显著相关。提示T—AK细胞释放的抑制性细胞因子产生的间接杀伤作用是其杀伤活性的构成成分之一。The antitumor effects of supernatant preparation, made from the culture media of T-activited killer(T-AK)cells costimulated with interleukin-2 (rIL-2)and anti-CD3 monoclonal antibody (CD3McAb) , on K562 (leukemia ) and BEL-7402 cells (hepatic carcinoma ) were studied, and the interrelationship between the cytolytic activity of T-AK cells and the tumor-inhibitory activity of T-AK supernatant was observed. It was found that (1). T-AK cells produced spontaneously some inhibitory factors which could inhibite the proliferation of K562 cells and BEL-7402 cells ,but the inhibitory activity was in a low level. (2) The tumor-inhibitory substances released from the process of T-AK cells killing K562 cells had a higher and nonspecific cytotoxicity on the tumor cells. (3). The cytolytic activity of T-AK cells had a positive interrelationship with the tumor-inhibitor activity of T-AK supernatant preparation. These results showed that the indirect cytotoxicity of inhibitory factors in T-AK supernatant was one of the antitumor mechanisms of T-AK cells.

关 键 词：T-AK细胞 上清液 杀伤活性 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤] R392.12[医药卫生—临床医学]