Ki67、Survivin、P16表达在宫颈癌和宫颈癌前病变诊断中的意义  

Expression and significance of Ki67, survivin and P16 in the diagnosis of cervix carcinoma and cervical intraepithelial neoplasia

作　　者：何慧仪[1] 卢丽娜[1] 杜洪[2] 朱秋云[1] 方艺川[1] 陈倩文[1] 

机构地区：[1]广州医学院附属广州市第一人民医院妇产科,510180 [2]广州医学院附属广州市第一人民医院病理科

出　　处：《中国妇幼保健》2006年第13期1825-1827,共3页Maternal and Child Health Care of China

基　　金：广州市医药卫生科技计划项目(2005-YB-007)

摘　　要：目的:研究Ki67、Survivin、P16在宫颈癌和宫颈癌前病变中的表达及生物学意义和早期诊断价值。方法:对160例高危病人行阴道镜检并在镜下活检宫颈组织,采用免疫组织化学SP法检测Ki67、Survivin、P16,并结合临床病理特点分析。结果:160例患者中有96例宫颈炎、40例宫颈内瘤样变、24例宫颈癌,Ki67阳性表达率分别为45.83%、95.00%、100.00%;Survivin阳性表达率分别为25.00%、90.00%、100.00%;P16阳性表达率分别为12.50%、60.00%、100.00%。Ki67、Survivin、P163种蛋白在宫颈内瘤样变、宫颈癌中的表达与宫颈炎相比有显著性差异(P<0.0001);Survivin、P16与Ki67蛋白表达间存在正相关(r=0.599;P<0.01;r=0.540,P<0.01)。结论:Ki67、Survivin、P16联合检测可作为早期诊断宫颈癌前病变及宫颈癌的标记物,可提高宫颈癌的早期诊断率。Objective： To investigate the expression, biological significance and value of Ki67, survivin and P16 in early diagnosis of the cervix carcinoma and the cervical intraepithelial neoplasia. Methods： The immunohistochemical SP was used to detect the expression of the Ki67, survivin and P16 proteins. For the 160 cases of high dangerous patients , the biopsy of the cervix had been done through the colposcopy to observe the relationship of the expression with clinical pathological variables. Results ： Of 160 cases, 96 cases of cervicitis, 40 cases of cervical intraepithelial neoplasia and 24 cases cervix carcinoma, the positive expression of the Ki67 in cervicitis, cervical intraepithelial neoplasia and cervix carcinoma were as below as 45. 83% , 95. 00% , and 100, 00%, The positive expression of survivin in cervicitis, cervical intraepithelial neoplasia and cervix carcinoma were as below as 25. 00% , 90. 00% , and 100. 00%, The positive expressions of P16 in cervicitis, cervical intraepithelial neoplasia and cervix carcinoma were as below as 12. 50% , 60. 00% , and 100, 00%, By all appearance, for the positive expression of these three kinds of proteins, the data in cervical intraepithelial neoplasia and in cervix carcinoma were significantly different from that one in cervicitis （ P 〈 0. 000 1 ） . There was a positive correlation among these three kinds of proteins （r =0. 599, P 〈0. 01 ; r =0. 54, P 〈0. 01 ） . Conclusion： Ki67, survivin and P16 could be used as markers for early diagnosis of cervix cancer. It could be helpful for increasing the ratio of earlier diagnosis in the fields of cervix cancer.

关 键 词：宫颈癌 宫颈癌前病变 KI67 SURVIVIN蛋白 P16蛋白 

分 类 号：R737.3[医药卫生—肿瘤]