基因重组乳链球菌防龋疫苗口服免疫孕兔的实验研究  

Oral immunization with recombinant S. lactis carrying the S. mutans surface protein antigen gene in pregnant rabbits

作　　者：刘建国[1] 樊明文[1] 边专[1] 凌均启 汪喻忠[1] 陈罕[1] 

机构地区：[1]武汉市湖北医科大学口腔医学院,遵义医学院口腔系

出　　处：《口腔医学纵横》1996年第4期195-198,共4页Journal of Comprehensive Stomatology

基　　金：国家自然科学基金

摘　　要：作者研究了重组链球菌ＨＬ１０７口服免疫孕兔的效果。使用酶联免疫吸附法测定口服免疫前后血清、唾液和乳汁中抗表面蛋白抗原ＩｇＧ、ＩｇＡ抗体水平。结果表明口服免疫孕兔后血清和乳汁中特异性抗表面蛋白抗原的ＩｇＧ水平明显升高，唾液和乳汁中抗表面蛋白抗原的ＩｇＡ水平明显升高，提示重组乳链球菌ＨＬ１０７具有变形链球菌表面蛋白抗原的免疫原性，能够激发针对表面蛋白质抗原的系统免疫和局部粘膜的免疫反应。The authors studied the effect of oral immunization in pregnant rabbits with recombinant S. lactis HL107.The level of anti-PAc IgG, IgA in milk, serum and saliva was measured with ELISA before and after oral immunization. The result indicated that specific anti-PAc IgG in serum and milk and specific anti-PAc IgA in saliva and milk were induced to high level after oral immunization. It suggests that the recombinant S. lactis HL107 possesses immunogenicity of 5 mutans surface protein and be able to develope specific systematic and local mucosa immune response againat PAc.

关 键 词：变形链球菌 乳链球菌 免疫学 疫苗 龋齿 

分 类 号：R781.101[医药卫生—口腔医学] R780.2[医药卫生—临床医学]