小儿恶性肿瘤突变型P_(53)蛋白的检测及临床意义  

The Detection of Mutant P_(53)in Malignant Tumors and Its Clinical Significance

作　　者：郑修军[1] 董蒨[1] 江布先[1] 黄婉芬[1] 

机构地区：[1]青岛医学院附属医院小儿外科,266003

出　　处：《中华小儿外科杂志》1996年第6期327-329,共3页Chinese Journal of Pediatric Surgery

基　　金：山东省教委资助课题

摘　　要：采用免疫组织化学方法检测小儿常见恶性肿瘤突变型 P_(53)蛋白的表达情况并探讨其临床意义。用 DO-1P_(53)单克隆抗体,LSAB 法测定45例小儿恶性肿瘤石蜡切片的 P_(53)表达。19例神经母细胞瘤3例阳性,均为Ⅳ期患儿,其中1例阳性为睾丸转移瘤而原发瘤阴性;16例肾母细胞瘤4例阳性,其中3例预后不良型均为阳性;10例恶性淋巴瘤5例阳性,均为Ⅲ、Ⅳ期患儿。结果表明突变型 P_(53)蛋白的表达与三种肿瘤的临床分期、组织学类型有关,可能为三种肿瘤转移、复发的原因,为小儿恶性肿瘤预后不良的一个重要指标。Objective:To detect the expression of mutant P_(53)in Children's malignant tumors by immunohistochemical method and to discuss its clinical significance.Method:45 polysine-coat- ed slides were stained with DO-1 P_(53)monoclonal antibodies by the method of LSAB.Results: There were 3 cases(stage Ⅳ)of positive mutant P_(53)in 19 neuroblastomas.Of them,one case of testicular metastatic tumor was positive,but the primary tumor was negative.Four cases(UF type in 3)of the 16 nephroblastomas were positive.Five of the 10 malignant lymphomas were pos- itive.All of them belonged to stage Ⅲ or Ⅳ.Conclusion:The expression of mutant P_(53)is related to clinical stage and histologic type of the above three tumors.It is probably a cause of tumor metastasis and relapse.The mutant P_(53)being positive appears to be an important prognostic factor of some tumors in children.

关 键 词：肿瘤 组织化学 免疫测定 P53蛋白 突变型 儿童 

分 类 号：R730.3[医药卫生—肿瘤] R730.45[医药卫生—临床医学]