逆转录病毒壳蛋白p15E在人肿瘤细胞的表达  

Expression of Retroviral Transmembrane Envelope Protein p15E in Human Malignant Cells

作　　者：罗利群[1] 袁维华[1] 田京燕 张友会[1] 

机构地区：[1]中国医学科学院,中国协和医科大学,北京肿瘤研究所,河南医科大学博士后流动站

出　　处：《中国医学科学院学报》1996年第6期416-422,共7页Acta Academiae Medicinae Sinicae

基　　金：国家"八五"科技攻关基金;河南医科大学博士后基金

摘　　要：肿瘤生长经常伴发免疫抑制。在其成因中有源于肿瘤的免疫抑制活性物质，但其性质大多未阐明。本文报道，采用免疫组化、酶联吸附、流式细胞、核酸原位杂交等技术，观察有免疫抑制活性的逆转录病毒壳蛋白ｐ１５Ｅ在人肿瘤细胞中不同水平的表达情况。结果揭示：肿瘤细胞表达ｐ１５Ｅ比较普遍，从人食管癌、鼻咽癌、直肠癌、卵巢癌（包括癌性腹水和建株细胞系）、胃癌、乳癌等均可被检出。ＲＴ－ＰＣＲ检测显示癌患者的活化淋巴细胞ｐ１５Ｅ表达略高。这些结果提示：来自肿瘤，也许还有来自活化淋巴细胞的ｐ１５Ｅ在肿瘤伴发的免疫抑制中可能起重要作用。Malignant tumor growth is always accompanied with imrnune suppression. Tumor-derived immuno-suppressive factors,among others,are thought to be responsible,but theirnature is not well defined.The paper reports on the expression of an immuno-suppressiveretroviral transmembrane envelope protein p15E at different levels in human cancer cells. Byusing immunohistochemistry,enzyme-linked immunosorbent assay,flow cytometry and insitu hybridization,p15E expression could be demonstrated in cancers of the esophagus,nosopharynx,rectum,ovary (including malignant effusion and cell lines),stomach andbreast.Enhanced expression of p15E was also observed in activated lymphocytes of cancerpatients as shown by RT-PCR technique. The results indicate that p15E of tumor origin,and perhaps that from activated lymphocytes,may contribute to the immune suppression associated with malignancy.

关 键 词：p15E CKS-17 逆转录病毒 壳蛋白 肿瘤 

分 类 号：R730.3[医药卫生—肿瘤] Q510.4[医药卫生—临床医学]