NO、TNF和IL-2与新型鸭肝炎病毒感染雏鸭肝脑组织损伤的关系  

Relationship Between the Dynamic Changes of NO,TNF,IL-2 and Tissue Hurt of Ducklings Infected with New Type Duck Hepatitis Virus

作　　者：胡薛英[1] 郑艳华[2] 王德海[2] 谷长勤[2] 苏敬良[1] 佘锐萍[1] 程国富[2] 

机构地区：[1]中国农业大学动物医学院,北京100094 [2]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国兽医学报》2006年第4期420-423,共4页Chinese Journal of Veterinary Science

基　　金：湖北省自然科学基金资助项目(2000J103);2004年度华中农业大学科技创新基金项目

摘　　要：用新型鸭肝炎病毒人工感染9日龄健康樱桃谷雏鸭，对感染后12、24、48、96、168h和14d雏鸭血液、肝和脑组织中一氧化氮（NO）含量，血液中肿瘤坏死因子（TNF）和白细胞介素2（IL-2）含量进行了测定，同时对感染雏鸭的组织病理学变化进行了观察。结果表明．血清中NO含量在接种后48h开始升高，一直持续到接种后96h，接种后7d恢复正常；肝脏组织中NO含量仅在接种后24h与对照组比较显著升高，在其他时间未表现有差异；脑组织中NO含量在整个试验期间没有变化。血清中的TNF和IL-2含量在接种后24h均表现升高，接种后96h降低，其他时间无改变。感染雏鸭的肝组织在接种后24h表现出血性坏死性肝炎变化，接种后48～96h呈增生性病变，而接种后各时期脑组织均呈非化脓性脑炎变化。由此表明，新型鸭肝炎病毒感染可导致雏鸭体内NO、TNF和IL-2发生变化，并且与肝组织损伤、疾病的发生发展有关。9-day-old ducklings were inoculated with new type duck hepatitis virus. And the NO in the serum, liver and brain, the TNF,IL-2 in the serum were determined at 12,24,48,72,96,168 h and 14 d post inoculation（PI）, and at the same time, the histopathological changes of liver and brain of inoculated duckling were observed. The result showed that the levels of serum NO increased significantly at 48,72 h and 96 h PI. The activities of liver NO began to increase significantly at 24 h PI. There were no changes in the content of brain NO. The concentration of serum TNF and IL-2 increased significantly at 24 h after inoculation, then dropped significantly at 96 h PI. Histopathological examination revealed the hemorrhagic and necrotic inflammation of the liver at 24 h PI. At 48 h to 96 h PI, proliferative inflammation was observed. All the time, the nonsuppuratire encephalitis was observed in the brain. The study implied that the duckling infected with new type duck hepatitis virus resuited in the changes of the concentration of NO, TNF and IL-2, and these changes were related to the hurt of the liver and the pathogenesis of the disease.

关 键 词：新型鸭肝炎病毒 一氧化氮 肿瘤坏死因子 白细胞介素-2 雏鸭 

分 类 号：S852.65[农业科学—基础兽医学] S858.32[农业科学—兽医学]