动物微生态制剂猪肠源乳球菌的分离与鉴定  

Isolation and Identification of the Lactococcus an Animal Micro-ecological Agent (AMEA) from the Intestines of Pig

作　　者：孟军[1] 刘淑英[1] 黄丽红 张燕[1] 

机构地区：[1]宁夏大学生命科学学院,宁夏银川750021 [2]平罗县畜牧发展事业局,宁夏平罗753400

出　　处：《微生物学杂志》2006年第3期12-15,共4页Journal of Microbiology

基　　金：宁夏大学科研基金开发项目

摘　　要：试验的菌种是从宁夏平罗县边远农村基本自然生长的健康肉猪的小肠、大肠和盲肠中分离获得的,共分离出64株菌株。通过对这64株菌株的菌落形态观察和革兰氏染色镜检。筛选出9株进行了乳球菌属的生理生化鉴定,初步确定这9株属于乳球菌属(Lactococcus)。再通过糖醇类发酵产酸鉴定,确定2株为乳酸乳球菌乳酸亚种(L.lactissubsplactis),3株为植物乳球菌(L.plantarum),4株为棉籽糖乳球菌(L.raffi-nolactis)。各项鉴定结果均符合乳球菌属和相应种鉴定标准。The bacterial species in this experiment were isolated from the small intestine, large intestine and blind intestine of a healthy pig raised in the conditional natural environment in the outlying countryside of Pingluo, Ningxia. 64 strains were isolated. Through the observation of colonial morphology and Gram staining, 9 strains were chosen and identified physiologically and biochemistrically according to the diagnostic characteristics of Lactococcus and were preliminarily defined as Lactococcus. Through the identification of fermentation and acid-producing of sugar and alco- hol, 2 strains were defined as L. lactis subsp lactis , 3 strains as L . plantarum , 4 strains as L. raffinolactis. All the results were coincidental to the identification criteria of genus and related species of Lactococcus.

关 键 词：乳球菌 生理生化鉴定 微生态制剂 益生菌 

分 类 号：S852.2[农业科学—基础兽医学]