PS1/APP双转基因阿尔茨海默病模型传代小鼠的基因型鉴定及其组织学分析  

Genetype identification and histologic analysis of PS1/APP double transgenic Alzheimer disease mouse model

作　　者：宋敏[1] 唐军[2] 李达兵[2] 徐海伟[2] 白云[1] 

机构地区：[1]第三军医大学基础医学部医学遗传学教研室,重庆400038 [2]第三军医大学基础医学部生理学教研室,重庆400038

出　　处：《第三军医大学学报》2006年第14期1453-1456,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金海外青年学者合作研究基金资助项目(30228018)~~

摘　　要：目的对所获的PS1/APP双转基因阿尔茨海默病(A lzhe im er d isease,AD)模型小鼠进行基因鉴定,进一步对其进行组织学分析,检测老年斑(sen ile p laque,SP)的形成情况。方法设计特定的引物,PCR扩增转入基因组DNA中的APP基因,对转入PS1/APP的小鼠应用刚果红染色结合免疫组化观察Aβ沉积、小胶质细胞和星型胶质细胞的活化。结果与未转入的阴性对照相比,在PS1/APP双转基因的AD小鼠皮质和海马内可见Aβ斑块形成,围绕在Aβ斑块周围的小胶质细胞和星形胶质细胞处于活化状态,形成典型的SP结构。结论我们获得的PS1/APP双转基因小鼠能够模拟AD患者脑内的主要病理过程,提供有效的实验动物模型。Objective To identify the genetype of the PS1/APP double transgenic mouse model, analyse the tissue histology and detect the formation of the senile plaque. Methods The specific primer was designed and the APP gene was amplified by PCR from the mouse genome DNA. The Aβ deposits, activation of microglia and astrocyte were observed by the congo red staining and immunohistologic methods. Results As compared to the negative control, the PS1/APP mouse displayed Aβ deposits restricted to cortical and hippocampal structures, the activated microglia and astrocyte surrounded the plaque, thus the classic senile plaque stucture was formed. Conclusion The double transgenic PS1/APP mouse produced by the two mice we bought from American Jackson Laboratory could simulate the specific pathogenesis of Alzheimer disease and be the efficient experimental animal model.

关 键 词：阿尔茨海默病 转基因小鼠 老年斑 

分 类 号：R394-33[医药卫生—医学遗传学] R394.2[医药卫生—基础医学]