射频消融对大鼠外周血及肝脏局部组织白细胞介素-10水平的影响  

Effect of radiofrequency ablation of normal rat liver on IL-10 in peripheral-blood and local area of liver

作　　者：戴维德[1] 范智慧[1] 陈敏华[1] 河福金[2] 李洪民[2] 

机构地区：[1]北京大学临床肿瘤学院北京肿瘤医院超声科,北京100036 [2]北京大学临床肿瘤学院北京肿瘤医院病理科,北京100036

出　　处：《中国医学影像技术》2006年第6期808-810,共3页Chinese Journal of Medical Imaging Technology

基　　金：北京市科委重大项目培育专项(Z0005190040431)。

摘　　要：目的探讨肝脏射频消融前后大鼠外周血及肝脏局部组织白细胞介素10(Interleukin10,IL10)水平的变化及意义。方法30只健康SD大鼠随机分为射频后1周组、射频后2周组和对照组,每组10只。射频后1周组及射频后2周组分别在射频后1周及2周处死,对照组不做射频即处死,取外周血及射频灶周边0.5cm范围内肝组织(对照组取正常肝组织),采用Ficoll密度梯度离心法分离外周血及射频灶周边肝组织单个核细胞,应用流式细胞术检测其IL10表达水平。结果对照组大鼠的外周血IL10表达水平为94.8333±3.5388。射频后1周为88.96±5.7230,射频后2周为63.08±13.7723,射频后2周组与对照组比较IL10表达水平降低,差异有显著性。射频后1周组与对照组比较差异没有显著性。对照组大鼠的肝组织IL10表达水平为94.9333±2.0817。射频后1周为93.3400±2.1652,射频后2周为72.9400±10.4677,射频后2周组与对照组比较IL10表达水平降低,差异有显著性。射频后1周组与对照组比较差异没有显著性。结论射频消融后大鼠外周血及射频灶周边肝组织IL10表达水平降低,推断RFA可能削弱IL10对树突状细胞分化成熟的抑制作用。Objective To study the effect of radiofrequency ablation of normal rat liver on IL-10 in peripheral-blood and local area of liver. Methods Thirty healthy SD rats were randomly separated into group 1 week after RFA, group 2 weeks after RFA and control group; there were ten rats in each group. Peripheral blood and liver tissue around the local area of control group, group 1 week after RFA and group 2 weeks after RFA were taken out respectively without RFA, 1 week and 2 weeks after RFA. The mononuclear cells of peripheral blood and liver tissue were separated by Ficoll density gradient centrifugation. The expression level of IL-10 in peripheral blood and liver tissue were analyzed with flowcytometry. Results The expression level of IL-10 in peripheral blood of control group was 94. 8333±3. 5388, and that of group 1 week and 2 weeks after RFA were respectively 88.96±5. 7230 and 63.08±13. 7723. The difference between control group and group 2 weeks after RFA was marked. The expression level of IL-10 in liver tissue of control group was 94. 9333：t：2. 0817, and that of local area around RFA focus of group 1 week and 2 weeks after RFA were respectively 93. 3400±2. 1652 and 72. 9400±10. 4677. The difference between control group and group 2 weeks after RFA was marked. Conclusion RFA can reduce the expression level of IL-10 in peripheral-blood together with local area of normal rat liver and may decrease inhibitory action of IL-10 on differentiation and maturation of dendritic cells to a certain extent.

关 键 词：射频消融 大鼠 肝脏 IL-10 

分 类 号：R575[医药卫生—消化系统] R445.1[医药卫生—内科学]