高胆红素血症新生儿输血传播病毒的基因检测  

Transfusion transmitted virus infection in the neonates

作　　者：王文祥[1] 熊爱华[2] 肖昕[3] 周晓光[4] 叶贞志[5] 李卫东[1] 

机构地区：[1]广东省中山市小榄人民医院儿科,528415 [2]暨南大学药学院实验技术中心 [3]暨南大学第一附属医院围产医学中心 [4]广州医学院第二附属医院新生儿科 [5]广东省深圳市宝安区妇幼保健院

出　　处：《中华传染病杂志》2006年第3期186-189,共4页Chinese Journal of Infectious Diseases

基　　金：广东省医学科学技术研究基金项目(A2002897)

摘　　要：目的探讨输血传播病毒(TTV)感染与新生儿高胆红素血症的相关性及肝功能受损情况,并分析TTV(广州株)开放阅读框(ORF)1区基因序列特征。方法对58例高直接胆红素(5例肝病综合征)、92例高间接胆红素血症和85例正常新生儿,应用套式聚合酶链反应(PCR)技术进行外周血TTV DNA扩增、电泳及序列分析,同时检测血清丙氨酸转氨酶(ALT)。结果高直接胆红素患儿中,7例外周血检测出TTV DNA(阳性率为12．1%),其中3例肝病综合征患儿TTV DNA阳性；高间接胆红素血症和正常新生儿中,分别检出1例TTV DNA阳性(阳性率分别为1．1%和1．2%)。高直接胆红素(包括肝病综合征)患儿的TTV DNA阳性率明显高于高间接胆红素患儿和正常新生儿,差异有统计学意义(P＜0．01)。TTV广州株与日本株N22比较,同源性达87．1%～97．8%,但存在着点突变。结论TTV感染可能是本组引起新生儿直接胆红素升高的原因之一,TTV广州株与日本株具有同源性。Objective To investigate the relationship between transfusion transmitted virus （TTV） infection and neonatal hyperbilirubinemia, the effect of TTV infection on the liver function, and analyse the feature of nucleotide sequences in TTV ORF1. Methods Serum TTV DNA, which were from 58 neonates with high direct bilirubin （DB, including 5 with hepatitis Syndrome）, 92 ones with high indirect bilirubin （IB）, and 85 normal ones, was detected using a nested polymerase chain reaction technique （nPCR）, electropherosis and sequence analyse, and serum alanine amniotransferase （ALT） was determined in all neonates. Results In DB neonates, TTV DNA were detected in 7 neonates （12.1%, including 3 neonates with hepatitis syndrome） ; in IB and normal ones, 1 neonate had positive TTV DNA （1.1% and 1.2 %）, respectively. Even if there were point mutations in Guangdong＇s TTV, the homology of Guangdong＇s TTV （GD1 9） and Japanese TTV （N22）ranged from 87. 1%～97.8% at nucleotide level. Conclusion TTV infection may be one of important pathogenesis resulting in neonatal hyperbilirubinemia and liver damage in such patients. Guangdong＇s and Japanese TTV isolates had the same genotype, some gene mutations maybe increase the pathogenicity in TTV.

关 键 词：输血传播病毒 黄疸 新生儿 病毒性疾病 序列分析 

分 类 号：R722.1[医药卫生—儿科]