益肝康药物血清对大鼠肝星状细胞胞浆游离钙效应的抑制作用  

Effects of Yigankang on Ca^(2+) in the Course of HSCs Activiting

作　　者：王天轶[1] 王军民[2] 王如美[3] 柳继兴[3] 姚希贤[4] 

机构地区：[1]白求恩国际和平医院急诊科,河北省石家庄市050000 [2]河北省人民医院消化科 [3]河北工程大学医学院 [4]河北医科大学附属第二医院消化科

出　　处：《中国全科医学》2006年第14期1172-1174,共3页Chinese General Practice

基　　金：河北省科技厅基金资助项目(02276103D-8)

摘　　要：目的研究益肝康药物血清是否具有抑制肝星状细胞(HSCs)胞浆游离钙([Ca2+]i)增高的作用。方法将32只健康雄性SD大鼠按随机数字表分为4组:肝纤维化模型益肝康组(8只)皮下注射用精制橄榄油配制的40%CCL4溶液9周,然后灌服益肝康6d;肝纤维化模型对照组(8只)皮下注射用精制橄榄油配制的40%CCL4溶液9周,然后灌服0·9%氯化钠溶液6d;正常大鼠益肝康组(8只)皮下注射等量精制橄榄油9周,然后灌服益肝康6d;正常对照组(8只)皮下注射等量精制橄榄油9周,然后灌服0·9%氯化钠溶液6d。结束后经下腔静脉取血并分离血清。用盲法,采用上述10%血清培养HSCs24h并负载好Fluo-3/AM后,使用激光扫描共聚焦显微镜(LSCM)检测HSCs[Ca2+]i。结果(1)经正常大鼠益肝康组及肝纤维化模型益肝康组大鼠血清预处理的HSCs,其[Ca2+]i荧光强度相对值均明显低于肝纤维化模型对照组(P<0·05);且两者与正常对照组间差别均有显著性意义(P<0·05)。结论益肝康能抑制肝星状细胞[Ca2+]i的升高,这可能是其发挥抗肝纤维化作用的重要途径之一。Objective To investigate whether pharmacological serum from the rats taking Yigankang can inhibit the increase of intracellular free calcium level in HSCs. Methods 32 health male rats were divided into 4 groups, hepatic fibrosis group was injected by 40% CCL4 subcutaneously for 9 weeks. Then Yigankang was filled into the stomachs of one medicated group but the same volume of 0. 9% NaCl into another medicated groups twice a day for 6 consecutive days. The same thing hap- pened in another two groups which were not modeled into hepatic fibrosis by 40% CCL4. The pharmacological serum was drawn from the inferior vena cava in two hours after the last time filling of drugs or NaCI on the morning of the 7th day. The serum drew from rats was dispensed in the 8% RPMI - 1640 culture medium to make the 10% drug serum RPMI - 1640 culture medium mixture, which was used to cultivate HSCs, Fluo -3 - loaded HSCs and examined effects of Yigankang on [ Ca^2＋ ] i with laser scanning confacal microscopy （LSCM）. Results In activated HSCs, both Yigankang pharmacological serums decreased [ Ca^2 ＋ ] i in HSCs significantly compared with controls （ P 〈 0. 05）. Conclusion Yigankang decreased [ Ca^2 ＋ ] i in activated HSCs, which may be one of important ways to block liver fibrosis.

关 键 词：肝纤维化 肝星状细胞 益肝康 激光扫描共聚焦显微镜 胞浆游离钙 

分 类 号：R575.2[医药卫生—消化系统] R285.5[医药卫生—内科学]