六级连续化膜生物反应工艺在丙烯酰胺生产中的应用研究  

Continuous bioconversion of acrylamide using a six-stage hollow-fiber membrane bioreactor

作　　者：孙旭东[1] 于慧敏[1] 罗晖[2] 沈忠耀[1] 

机构地区：[1]清华大学化学工程系生物化工研究所,北京100084 [2]北京科技大学环境工程系,北京100083

出　　处：《现代化工》2006年第7期45-48,共4页Modern Chemical Industry

基　　金：国家自然科学基金青年基金资助项目(20206014);全国百篇优秀博士学位论文作者专项(200345)资助

摘　　要：以自由细胞替代固定化细胞,成功设计了六级连续化中空纤维膜生物反应器新工艺,应用于丙烯酰胺的微生物转化过程,并对其操作工艺和可行性进行了研究。结果表明:在15℃下,该六级连续化过程稳定运行80 h时,丙烯腈的转化率达99.9%以上,丙烯酰胺产物浓度达434.23 g/L,生产效率达到0.01526 mol/(L.min),生产效率比固定化细胞批式反应提高了一倍以上,产品溶液浓度提高50%以上。中空纤维膜可以使反应液和菌体得到有效的分离,且在反应液中没有检测到副产物丙烯酸。新工艺实现了丙烯酰胺的稳定连续化生产。A new process of six-stage hollow-fiber membrane bioreaction system was designed for microbial continuous bioconversion of acrylamide from acrylonitrile, in which free cells of Nocardia was used to substitute the immobilized ones. The operating feasibility of the new system was studied. The results showed that the conversion rate of acrylonitrile, the concentration of acrylamide in the output solution and the final productivity reached 99.9%, 434.23 g/L and 0.01526 mol/（L·min）, respectively, after steady running for 80h at 15℃, resulting in one-fold increase of productivity and 50% increase of product concentration compared with the traditional batch biocatalysis technique using immobilized cells as biocatalysts. Further studies showed that the product acrylamide could be separated well from the free cells with no by-product, acrylate acid, which ensured a favorable foreground of the new technique for industrial application.

关 键 词：自由细胞 丙烯酰胺 微生物连续转化 中空纤维膜生物反应器 

分 类 号：TQ225.261[化学工程—有机化工] TQ920.6[轻工技术与工程—发酵工程]