手术终止妊娠对早孕妇女外周血中游离胎儿DNA的影响  

The affecting to fetal cell-free DNA in maternal plasma after first-trimester termination of pregnancy.

作　　者：钱莉芸[1] 郑梅玲[1] 琚竹梅[1] 王刚华[1] 

机构地区：[1]山西医科大学第一医院遗传研究室,太原030001

出　　处：《中国优生与遗传杂志》2006年第7期5-6,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的 检测在孕早期手术终止妊娠是否会对母体外周血浆中的胎儿DNA水平造成影响。方法选择孕6～9w妇女36例（自愿要求终止妊娠，无禁忌症，胎儿性别为男性），在其终止妊娠前后分别采取外周静脉血5ml。对其血浆中的游离胎儿DNA的SRY基因行荧光定量分析。结果在36例血浆标本中，术前有31例检测到胎儿DNA，术后全部检测到胎儿DNA。其浓度平均分别为62．40±21，70（31．80～128，00Eq／m1），101．04±37．40（58．20～209．70Eq／m1），术前与术后胎儿DNA含量有统计学差异（P〈0．01）。结论①孕妇外周血中游离胎儿DNA最早在孕45天即可检测到。②外周血中游离胎儿DNA的水平在妊娠终止后很快升高。③孕妇外周血中的游离胎儿DNA很可能来源于母胎之间出血。Objective ： To determine if first-trimester elective termination of pregnancy affects cell-free fetal DNA levels in maternal plasma. Methods： Peripheral blood was collected from 36 pregnants （6-9weeks） at pre-termination and post-termination respectively , plasma was isolated immediately. Fetal DNA in maternal plasma was measured using real-time Fluorescence Quantitative Polymerase Chain-reaction （ FQ-PCR） for the SRY gene on the Y-chromosome. Results ： We detected fetal DNA in pre-termination samples from 31 out of 36 patients carrying male fetuses and in post-termination all samples were detected. The mean-concentrations in pre- termination and post-termination were 62.40±21.70 （31.80±128.00 Eq/ml） , 101.04±37.40 （58.20±209. 70 Eq/ml） respectively. There were significant differences between them （ P 〈0.01 ）. Conclusion ： （1） Fetal DNA was detected as early as 45 days of gestation. （2）The level of fetal DNA was increased immediately after termination of pregnancy in the first-trimester. （3）Increased fetal DNA may reflect fetomaternal hemorrhage or destruction of trophoblastic villi.

关 键 词：胎儿DNA 早期手术终止妊娠 SRY基因 母胎出血 

分 类 号：R169.42[医药卫生—公共卫生与预防医学]