B7-2分子在肝硬化患者肝脏组织中表达的Western blot检测方法研究  

An analysis of Western blot for detection of B7-2 protein in liver of patient with hepatic cirrhosis

作　　者：杨权[1] 钟备[1] 高翔[2] 殷国庆[1] 

机构地区：[1]东南大学医学院附属南京第二医院传染科,江苏南京210003 [2]南京大学模式动物研究所,江苏南京210061

出　　处：《东南大学学报（医学版）》2006年第4期259-261,共3页Journal of Southeast University(Medical Science Edition)

基　　金：江苏省卫生厅资助项目(TS-9904);南京市医学卫生专项基金资助项目(ZGG1999)

摘　　要：目的:建立肝硬化患者肝组织B7-2分子W estern b lot检测方法。方法:设置不同的一抗稀释比,筛选合适一抗稀释比。设置阳性对照、阴性对照,确认目的蛋白。结果:在一抗稀释比从1∶50到1∶200的范围内,蛋白显影灰度逐渐增加,随着一抗稀释比从1∶200到1∶1 000的进一步递减,蛋白显影灰度却逐渐减弱。结论:在一抗稀释比为1∶200条件下,B7-2分子在肝硬化患者肝脏组织中呈强阳性表达。Objective To set up the method of Western blot for detection B7-2 protein in liver of patients with hepatic cirrhosis. Methods Western blot was used. The optimum dilution was determined by anlyzing protein grayscale in different B7-2 antibody dilutions. The B7-2 protein was confirmed by comparing with positive controls and negative controis. Result The developing grayscale of B7-2 protein in liver of human was increased in the range of 1 ： 50 to 1 ： 200, but decreased in the range of 1：200 to 1：1 000. Conclusion The B7-2 protein is strongly expressed in liver of the patients compared with the positive and negative controls under the condition of 1：200 B7-2 antibody dilution.

关 键 词：B7—2蛋白 WESTERN BLOT 肝硬化 

分 类 号：R575.2[医药卫生—消化系统]