海珠益肝胶囊对免疫性肝损伤小鼠Th1/Th2细胞的调节作用  

Effect of HaiZhuYiGan Capsule on Th1/Th2 Cells in Mouse with Immunological Liver Injure

作　　者：黄育华[1] 晏雪生[1] 彭亚琴[1] 明安萍[1] 盛国光[1] 

机构地区：[1]湖北中医学院附属医院脏象肝病研究所,湖北武汉430061

出　　处：《中医药学刊》2006年第7期1240-1242,共3页Study Journal of Traditional Chinese Medicine

基　　金："九五"国家重点科技项目(96-906-08-01)

摘　　要：目的:探讨海珠益肝胶囊对免疫性肝损伤小鼠外周血Th1/Th2细胞平衡的调节作用。方法:应用尾静脉注射卡介苗(BCG)和脂多糖(LPS)建立免疫性肝损伤小鼠模型,分离小鼠淋巴细胞,经佛波醇乙酯(PMA)、离子霉素、莫能菌素刺激培养6h后,收获细胞,利用细胞内细胞因子染色流式细胞术检测Thl/Th2比例;另外经刀豆蛋白A(ConA)刺激培养72h后,收集培养上清,ELISA法检测IL-2、IFN-γ、IL-4、IL-10的水平。结果:在模型组,小鼠外周血PBMC中Th1型细胞因子IL-2、IFN-γ水平升高,Th2型细胞因子IL-4、IL-10水平降低,与正常对照组比较有显著性差异(P<0.05);Th1细胞的百分率升高,Th2细胞的百分率降低,Th1/Th2的比值增大,与肝组织损害程度呈现正相关。海珠益肝胶囊能够抑制Th1型细胞因子的分泌,降低Th1细胞的百分率,恢复Th1/Th2的比例,对肝组织损害具有保护和改善作用(P<0.05)。结论:Th1/Th2细胞失衡是造成小鼠免疫性肝损伤的原因之一,Th1应答占主导地位,海珠益肝胶囊能够调节Th1/Th2细胞平衡,对肝组织损害具有保护和改善作用,可能是其免疫药理机制之一。Objective： To investigate the effects of HaiZhuYiGan Capsule on Th1/Th2 ceils in the peripherl blood mononuclear cells from the mouse with immunological liver injure. Methods： The ratios of Th1/Th2 ceils in the mouse with immunological liver injure were detected by flow cytometry with two color intracellular cytokine staining;The levels of IL- 2, IFN -γ、IL- 4 and IL- 10 were detected by ELISA. Results： In the model group, the levels of IL-2. IFN-T were higher, the levels of IL- 4, IL- 10 were lower, Th1 cell ratios were higher, Th2. cell ratios were lower, the ratios of Th1/Th2 were higher, there was positive correlative between the ratio of Th1/Th2 and liver injure. HaiZhuYiGan Capsule could downregulate the levels of IL -2, IFN - γ and the radio of Th1 cell, and could convert the ratio of Th1/Th2, and could improve liver pathological injury（ P 〈 0.05）. Conclusion： The imbalance of Th1/Th2 was one of the causes of mouse immunological liver injure, Th1 cells were predominate, HaiZhuYiGan Capsule could convert the imbalance of Th1/Th2 and make it incline to the normal state, and improve liver pathological injury.

关 键 词：海珠益肝胶囊 TH1细胞 TH2细胞 流式细胞术 免疫性肝损伤 

分 类 号：R228[医药卫生—中医基础理论]