雷公藤多甙对佐剂性关节炎大鼠自由基和炎症因子的影响  

Influence of tripterygium po1yglycoside on free radicals and inflammatory factors in rats with adjuvant arthritis

作　　者：肖诚[1,2] 何颖辉[3] 黄芳华[4] 吕诚[5] 赵林华[5] 赵宏艳[5] 周静[5] 藤静如[5] 吕爱平[5,6] 

机构地区：[1]中日友好医院临床研究所 [2]中国中医科学院基础理论研究所北京100700 [3]江西中医学院国家工程研究中心 [4]中国药科大学 [5]中国中医科学院基础理论研究所 [6]江西中医学院国家工程研究中心江西330077

出　　处：《北京中医药大学学报》2006年第6期389-392,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30171133;No.39870952)

摘　　要：目的探讨雷公藤多甙对佐剂性关节炎(AA)大鼠的保护作用及可能机制。方法采用大鼠AA模型,将30只SD雄性大鼠随机分为3组即正常组、模型组、雷公藤多甙组。对足爪肿胀进行测量,在光镜下观察各组组织形态学变化,生化方法检测了动物血清超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量以及总抗氧化能力(T-AOC)。用放射免疫法检测了足爪TNF-α及PGE2含量。用免疫组织化学方法检测了膝关节软骨环氧化酶-2(COX-2)及核因子κB(NF-κB)表达。结果与正常组相比,模型组血清SOD和T-AOC能力下降(P<0·01),MDA含量升高;足爪TNF-α及PGE2含量升高;膝关节软骨COX-2及NF-κB表达增加。雷公藤多甙组能升高血清SOD和T-AOC活力,降低血清MDA、足爪TNF-α、PGE2含量及膝关节软骨COX-2及NF-κB表达。光镜下组织形态观察表明雷公藤多甙组能减轻滑膜增生,减少炎细胞浸润。结论雷公藤多甙对AA模型具有保护作用,抗氧化能力及抑制炎症细胞因子浸润可能是其主要的作用机制之一。Objective To investigate the protective effect and mechanism of tripterygium pol yglycoside （TII） on rats with adjuvant arthritis （AA）. Methods The rat AA model was established and 30 SD male rats were divided randomly into the normal group, model group and TII group. The swollen of rat claw were measured, the histological changes were observed by the light microscope, the activity of superoxide dismulase （SOD）, MDA level and T-AOC were detected by the biochemical method, the contents of TNF-α and PGE2 in claw were detected by the radioimmunoassay, and the expressions of COX-2 and NF-KB in cartilage of knee joint were detected by immunohistoehemistry. Results Compared with the normal group in the model group the activities of SOD and T-AOC were decreased （P 〈 0.01 ）, MDA level was increased, the contents of TNF-ct and PGE2 were inereasod, and the expressions of COX- 2 and NF-KB were increased. In the TII group the activities of SOD and T-AOC were increased, the MDA level, the contents of TNF-ct and PGE2, and the expressions of COX-2 and NF-κB were decreased. The result of the light microscope showed that in the TII group the hyperplasia of synovial membrane and inflammatory infiltration was relieved. Conclusion Tripterygium pol yglyeoside has a protective effect on rat AA model. One of its mechanisms may be related to its antioxidation and inhibitory effects on inflammatory infiltration.

关 键 词：雷公藤多甙 佐剂性关节炎 自由基 炎症因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]