浊点系统中青霉素水解物系的分配及固定化青霉素酰化酶的稳定性  

Partitioning of penicillin G hydrolysis reactants and stability of immobilized penicillin acylase in cloud point system

作　　者：郭雅静[1] 王志龙[1] 包达 齐瀚实[1,3] 

机构地区：[1]上海交通大学药学院 [2]上海海湃生物科技有限公司 [3]上海交通大学生物技术研究所,上海201101

出　　处：《化工学报》2006年第6期1422-1425,共4页CIESC Journal

摘　　要：Enzymatic hydrolysis of penicillin G(Pen G)into phenylacetic acid(PAA)and 6-aminopenicillanic acid(6-APA)suffers not only substrate inhibition and non-competitive inhibition by 6-APA,but also competitive inhibition by PAA.In the industrial process,PAA is generally eliminated by addition of aqueous ammonia to form soluble ammonium salt,which generates industrial waste.Extractive biocatalysis is a potential method to overcome the above obstacles and might be even directly used in enzymatic hydrolysis of penicillin G from fermentation broth.The partitioning behavior of Pen G hydrolysis reactants and the stability of immobilized penicillin acylase in cloud point system,a novel extractive bioreaction system,were determined.It suggests that cloud point system might be a novel extractive bioreaction system for the industrial process of penicillin hydrolysis.Enzymatic hydrolysis of penicillin G （Pen G） into phenylacetic acid （PAA） and 6- aminopenicillanic acid （6-APA） suffers not only substrate inhibition and non-competitive inhibition by 6- APA, but also competitive inhibition by PAA. In the industrial process, PAA is generally eliminated by addition of aqueous ammonia to form soluble ammonium salt, which generates industrial waste. Extractive biocatalysis is a potential method to overcome the above obstacles and might be even directly used in enzymatic hydrolysis of penicillin G from fermentation broth. The partitioning behavior of Pen G hydrolysis reactants and the stability of immobilized penicillin acylase in cloud point system, a novel extractive bioreaction system, were determined. It suggests that cloud point system might be a novel extractive bioreaction system for the industrial process of penicillin hydrolysis.

关 键 词：浊点系统 青霉素水解 青霉素酰化酶 分配 稳定性 

分 类 号：TQ923[轻工技术与工程—发酵工程]