P33ING1在肛管癌中的表达及与P53和细胞凋亡的关系  

Expression of P33ING1,P53 and their relationship with apoptosis in anal canal carcinoma

作　　者：陈利生[1] 李天煜[1] 曹云飞[1] 梁君林[1] 张森唐 卫中[1] 唐宗江[1] 高枫[1] 

机构地区：[1]广西医科大学第一附属医院结直肠肛门外科,南宁530021

出　　处：《中华胃肠外科杂志》2006年第4期338-341,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：广西科学基金(桂科回0342018);广西卫生厅重点项目(重200206)

摘　　要：目的探讨P33ING1在肛管癌中的表达及与P53和细胞凋亡的关系。方法应用免疫组织化学SP法检测42例肛管癌标本中P33ING1和P53的表达,同时检测其细胞凋亡,并与肛管腺瘤和乳头状瘤及肛周炎性肿块对照组比较。结果在肛管癌、肛管腺瘤及乳头状瘤、肛周炎性肿块组织中P33ING1阳性表达率分别为40．5%、97．2%及97．5%；P53分别为50．O%、22．2%及27．5%；平均细胞凋亡指数则分别为(10．27±1．23)‰、(42．67±1．04)‰及(42．75±0．98)‰。肛管癌中P33ING1、P53阳性率及平均细胞凋亡指数与另外两组比较,差异有统计学意义(P＜0．05)。在P53表达阳性的21例病例中有18例P33ING1表达阴性。结论P33ING1在肛管癌组织中低表达,对肛管癌的发生、发展可能起重要作用。P33ING1和P53互相协同,抑制细胞生长、促进调亡。Objective To explore the expressions of P33ING1, P53 and their relationships with apoptesis in anal canal carcinoma （ACC） . Methods The expressions of P33ING1, P53 proteins were measured by immunohistechemistry method（SP method）, and apoptesis was detected in 42 cases with ACC, 36 cases with anal canal adenoma （ACA） or anal canal papilloma （ACP）, and 40 cases with paraanal inflammatory mass（PAIM） . Results The positive expression rates of P33ING1 and P53 proteins were 40. 5% （17/42）, 97.2% （35/36） and 97. 5% （39/40）, 50.0% （21/42）, 22. 2% （8/36） and 27.5% （11/40） respectively, and the average apoptesis indexes（AI） were （10. 27± 1.23）Kv, （42. 75±0. 98）‰ and （42. 67±1.04）‰ respectively in ACC, ACA or ACP and PAIM. There were significant differences in the positive expression rates of P33ING1, P53 and apoptosis index between ACC and the other two groups respectively （ P 〈 0. 05） . Among 21 cases of ACC with positive expression of P53 protein, there were 18 cases with P33ING1 negative expression. Conclusions P33ING1 expression decrease in ACC, which may play an important role in the carcinogenesis and progression of ACC. P33ING1 and P53 may have an synergistic effect of suppressing cell growth and accelerating cell apoptesis.

关 键 词：肛管肿瘤 P33ING1 P53 细胞凋亡 免疫组织化学法 

分 类 号：R735.38[医药卫生—肿瘤]