CpG岛甲基子表型阳性的散发性大肠癌的临床病理特征  

Clinicopathologic features of sporadic colorectal cancers with CpG island methylator phenotype

作　　者：蔡国响[1] 蔡三军[1] 徐烨[1] 师英强[1] 孙孟红[2] 陆洪芬[2] 管祖庆[1] 廉朋[1] 彭俊杰[1] 周晓燕[2] 杜祥[2] 施达仁[2] 

机构地区：[1]复旦大学附属肿瘤医院腹部外科,上海200032 [2]复旦大学附属肿瘤医院病理科,上海200032

出　　处：《中华消化杂志》2006年第6期377-380,共4页Chinese Journal of Digestion

基　　金：上海市卫生局科技发展基金(024072)。

摘　　要：目的探讨CpG岛甲基子表型阳性的散发性大肠癌的临床病理特征。方法采用甲基化特异性PCR方法对71例散发性大肠癌患者行p14、hMLH1、p16、MGMT和MINT1共5个基因启动子甲基化检测,分析CpG岛甲基子表型阳性的散发性大肠癌的临床病理特征。结果 71例散发性大肠癌患者中共检出CpG岛甲基子表型阳性者15例,阳性率为21．1%。CpG岛甲基子表型阳性的散发性大肠癌中右半结肠癌(40．0％比12．5%,P<0．05)、低分化癌(46．7％比14．3％,P<0．05)、淋巴结转移 (86．7％比48．2％,x2=7．112．P<0．05)、Dukes C期或D期(86．7％比50．0％,x2=6．519,P<0．05) 所占比例均显著高于CpG岛甲基子表型阴性者。结论 CpG岛甲基子表型阳性的散发性大肠癌具有右半结肠癌多发、低分化多见、常有淋巴结转移和分期较晚的特点。Objective To investigate the clinicopathologic features of sporadic colorectal cancers with CpG island methylator phenotype. Methods The methylation of the promotors of the five genes including p14, hMLIql, p16, MGMT and MINT1 were detected using methylation specific PCR in 71 patients to determine the clinicopathologic characteristics of sporadic colorectal cancers with CpG island methylator phenotype. Results Fifteen out of 71 （21. 1%） patients were positive for the CpG island methylator phenotype. The proportion of the right-sided colonic cancers（40.0% vs. 12.5%, P〈0.05）, poorly-differentiated cancers（46.7% vs. 14.3%, P〈0.05）, cancers with lymph node metastasis（86.7% vs. 48.2%, P^0.05） and cancers with Dukes C or D stage （86.7% vs. 50.0%, P^0.05）in sporadic colorectal cancers with CpG island methylator phenotype were significantly higher than those without CpG island methylator phenotype. Conclusions Compared with sporadic colorectal cancers without CpG island methylator phenotype, those with CpG island methylator phenotype tend to occur in the right hemicolon, to have poor differentiation and lymph node metastasis, and to be in the later stages.

关 键 词：结直肠癌 散发性 甲基化 CpG岛甲基子表型 

分 类 号：R735.34[医药卫生—肿瘤]